| Literature DB >> 15926814 |
Changlin Tian1, Richard M Breyer, Hak Jun Kim, Murthy D Karra, David B Friedman, Anne Karpay, Charles R Sanders.
Abstract
The seven-transmembrane-spanning G protein-coupled receptor (GPCR) superfamily plays many important roles in basic biology, human health, and human disease. Here, well-resolved solution NMR spectra are presented for a human GPCR, the vasopressin V2 receptor in detergent micelles. The quality of the NMR spectra indicates that backbone resonance assignments for a majority of resonances are feasible. The key to obtaining high quality spectra appears to be the coupling of methods for expressing the receptor into membranes rather than into inclusion bodies, with use of a biochemically mild lysolipid detergent for membrane extraction, protein purification, and NMR sample preparation.Entities:
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Year: 2005 PMID： 15926814 DOI： 10.1021/ja051161b
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419