Literature DB >> 15925542

Emerging technologies for the detection and genetic characterization of protozoan parasites.

Paul T Monis1, Steven Giglio, Alexandra R Keegan, R C Andrew Thompson.   

Abstract

The development and adaptation of new technologies for the genetic characterization and identification of parasites continue to accelerate, providing an increasing number of research and analytical tools. We review emerging technologies that have applications in this area, including real-time PCR and microarrays, and discuss the fundamental principles of some of these technologies and how they are applied to characterize parasites. We give special consideration to the application of genetic data to biological questions, where selection of the most appropriate technique depends on the biological question posed by the investigator.

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Year:  2005        PMID: 15925542     DOI: 10.1016/j.pt.2005.05.012

Source DB:  PubMed          Journal:  Trends Parasitol        ISSN: 1471-4922


  11 in total

1.  Quantitative detection and differentiation of free-living amoeba species using SYBR green-based real-time PCR melting curve analysis.

Authors:  Jonas Behets; Priscilla Declerck; Yasmine Delaedt; Lieve Verelst; Frans Ollevier
Journal:  Curr Microbiol       Date:  2006-11-13       Impact factor: 2.188

2.  Identification of forensically important blowfly species (Diptera: Calliphoridae) by high-resolution melting PCR analysis.

Authors:  Tadeusz Malewski; Agnieszka Draber-Mońko; Jan Pomorski; Marta Łoś; Wiesław Bogdanowicz
Journal:  Int J Legal Med       Date:  2010-01-16       Impact factor: 2.686

3.  Molecular diagnosis of the tick-borne pathogen Anaplasma marginale in cattle blood samples from Nigeria using qPCR.

Authors:  Nusirat Elelu; Joana Ferrolho; Joana Couto; Ana Domingos; Mark C Eisler
Journal:  Exp Appl Acarol       Date:  2016-10-27       Impact factor: 2.132

4.  An evaluation of primers amplifying DNA targets for the detection of Cryptosporidium spp. using C. parvum HNJ-1 Japanese isolate in water samples.

Authors:  Anna Susanne Leetz; Isaia Sotiriadou; Jerry Ongerth; Panagiotis Karanis
Journal:  Parasitol Res       Date:  2007-05-19       Impact factor: 2.289

Review 5.  Biotechnological advances in the diagnosis of little-known parasitoses of pets.

Authors:  Donato Traversa; Domenico Otranto
Journal:  Parasitol Res       Date:  2008-12-06       Impact factor: 2.289

6.  Identification of species by multiplex analysis of variable-length sequences.

Authors:  Filipe Pereira; João Carneiro; Rune Matthiesen; Barbara van Asch; Nádia Pinto; Leonor Gusmão; António Amorim
Journal:  Nucleic Acids Res       Date:  2010-10-04       Impact factor: 16.971

7.  Molecular epidemiology of Theileria equi in horses and their association with possible tick vectors in the state of Rio de Janeiro, Brazil.

Authors:  Maristela Peckle; Marcus S Pires; Tiago M Dos Santos; Erica C R Roier; Claudia B da Silva; Joice A R Vilela; Huarrisson A Santos; Carlos L Massard
Journal:  Parasitol Res       Date:  2013-03-09       Impact factor: 2.289

8.  An Evaluation of Quantitative PCR Assays (TaqMan® and SYBR Green) for the Detection of Babesia bigemina and Babesia bovis, and a Novel Fluorescent-ITS1-PCR Capillary Electrophoresis Method for Genotyping B. bovis Isolates.

Authors:  Bing Zhang; Jacqueline L Sambono; Jess A T Morgan; Bronwyn Venus; Peter Rolls; Ala E Lew-Tabor
Journal:  Vet Sci       Date:  2016-09-13

Review 9.  Next-generation molecular-diagnostic tools for gastrointestinal nematodes of livestock, with an emphasis on small ruminants: a turning point?

Authors:  Florian Roeber; Aaron R Jex; Robin B Gasser
Journal:  Adv Parasitol       Date:  2013       Impact factor: 3.870

Review 10.  Pathogen and Particle Associations in Wastewater: Significance and Implications for Treatment and Disinfection Processes.

Authors:  C Chahal; B van den Akker; F Young; C Franco; J Blackbeard; P Monis
Journal:  Adv Appl Microbiol       Date:  2016-09-15       Impact factor: 5.515

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