BACKGROUND/AIMS: Microglia are the primary antigen presenting cells in the central nervous system and the retina, and can harbour viral antigens that may damage neural tissue via the release of neurotoxins. All cells bearing CD4 molecules and co-receptors (members of the chemokine receptor and Fcgamma receptor families) are potential targets for the human immunodeficiency virus (HIV). In this study, retinal microglia (in vitro and in situ) were investigated for the expression of candidate HIV-1 binding receptors. METHODS: Cultured human retinal microglia and frozen sections of human retinas were used. Immunohistochemistry was used to investigate expression of cell surface receptors necessary for HIV-1 infection: CD4, CC chemokine receptor 5 (CCR5), and Fcgamma receptors. RESULTS: Human retinal microglia expressed detectable levels of CD4, CD16, CD64, and CCR5 in vitro and Fcgamma receptor I (CD64) in situ. CONCLUSIONS: Human retinal microglia express several candidate receptors required for viral binding and as such may be a potential reservoir for HIV-1 infection.
BACKGROUND/AIMS: Microglia are the primary antigen presenting cells in the central nervous system and the retina, and can harbour viral antigens that may damage neural tissue via the release of neurotoxins. All cells bearing CD4 molecules and co-receptors (members of the chemokine receptor and Fcgamma receptor families) are potential targets for the human immunodeficiency virus (HIV). In this study, retinal microglia (in vitro and in situ) were investigated for the expression of candidate HIV-1 binding receptors. METHODS: Cultured human retinal microglia and frozen sections of human retinas were used. Immunohistochemistry was used to investigate expression of cell surface receptors necessary for HIV-1 infection: CD4, CC chemokine receptor 5 (CCR5), and Fcgamma receptors. RESULTS:Human retinal microglia expressed detectable levels of CD4, CD16, CD64, and CCR5 in vitro and Fcgamma receptor I (CD64) in situ. CONCLUSIONS:Human retinal microglia express several candidate receptors required for viral binding and as such may be a potential reservoir for HIV-1 infection.
Authors: H A Gelbard; H S Nottet; S Swindells; M Jett; K A Dzenko; P Genis; R White; L Wang; Y B Choi; D Zhang Journal: J Virol Date: 1994-07 Impact factor: 5.103
Authors: A A Sadun; J S Pepose; M C Madigan; K A Laycock; W N Tenhula; W R Freeman Journal: Graefes Arch Clin Exp Ophthalmol Date: 1995-07 Impact factor: 3.117
Authors: D'Anne S Duncan; William M McLaughlin; Noah Vasilakes; Franklin D Echevarria; Cathryn R Formichella; Rebecca M Sappington Journal: J Clin Cell Immunol Date: 2017-05-24
Authors: D'Anne S Duncan; Rebecca L Weiner; Carl Weitlauf; Michael L Risner; Abigail L Roux; Emily R Sanford; Cathryn R Formichella; Rebecca M Sappington Journal: Front Neurosci Date: 2018-10-12 Impact factor: 4.677