Literature DB >> 15921966

Determination of intracellular concentrations of the TRPM2 agonist ADP-ribose by reversed-phase HPLC.

Andreas Gasser1, Andreas H Guse.   

Abstract

Since the NAD metabolite ADP-ribose (ADPR) has recently gained attention as a putative messenger, a method was established for the quantification of intracellular ADPR by reversed-phase HPLC. Cellular nucleotides were extracted with trichloroacetic acid, and crude cell extracts purified by solid phase extraction using a strong anion exchange matrix. After optimization of the extraction procedure, cellular ADPR levels were determined using two different reversed-phase columns (C18 versus C12), operated in ion pair mode. Intracellular ADPR concentrations in human Jurkat T-lymphocytes and murine BW5147 thymocytes were determined to be 44+/-11 microM and 73+/-11 microM, respectively.

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Year:  2005        PMID： 15921966     DOI： 10.1016/j.jchromb.2005.05.002

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


  11 in total

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Journal:  Biochem J       Date:  2006-09-01       Impact factor: 3.857

3.  Quantification of endogenous sirtuin metabolite O-acetyl-ADP-ribose.

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Journal:  Anal Biochem       Date:  2008-09-07       Impact factor: 3.365

4.  Structure and function of an ADP-ribose-dependent transcriptional regulator of NAD metabolism.

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Journal:  Structure       Date:  2009-07-15       Impact factor: 5.006

Review 5.  High-performance liquid chromatography-tandem mass spectrometry in the identification and determination of phase I and phase II drug metabolites.

Authors:  M Holcapek; L Kolárová; M Nobilis
Journal:  Anal Bioanal Chem       Date:  2008-03-15       Impact factor: 4.142

6.  8-Bromo-cyclic inosine diphosphoribose: towards a selective cyclic ADP-ribose agonist.

Authors:  Tanja Kirchberger; Christelle Moreau; Gerd K Wagner; Ralf Fliegert; Cornelia C Siebrands; Merle Nebel; Frederike Schmid; Angelika Harneit; Francesca Odoardi; Alexander Flügel; Barry V L Potter; Andreas H Guse
Journal:  Biochem J       Date:  2009-07-29       Impact factor: 3.857

7.  Function of BRCA1 in the DNA damage response is mediated by ADP-ribosylation.

Authors:  Mo Li; Xiaochun Yu
Journal:  Cancer Cell       Date:  2013-05-13       Impact factor: 31.743

8.  AtNUDT7, a negative regulator of basal immunity in Arabidopsis, modulates two distinct defense response pathways and is involved in maintaining redox homeostasis.

Authors:  Xiaochun Ge; Guo-Jing Li; Sheng-Bing Wang; Huifen Zhu; Tong Zhu; Xun Wang; Yiji Xia
Journal:  Plant Physiol       Date:  2007-07-27       Impact factor: 8.340

9.  Poly(ADP-ribose)glycohydrolase is an upstream regulator of Ca2+ fluxes in oxidative cell death.

Authors:  C Blenn; P Wyrsch; J Bader; M Bollhalder; Felix R Althaus
Journal:  Cell Mol Life Sci       Date:  2010-09-29       Impact factor: 9.261

Review 10.  Adenine Dinucleotide Second Messengers and T-lymphocyte Calcium Signaling.

Authors:  Insa M A Ernst; Ralf Fliegert; Andreas H Guse
Journal:  Front Immunol       Date:  2013-08-29       Impact factor: 7.561
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