OBJECTIVE: Although the epitopic breadth of HIV-1-specific CD8 T lymphocyte (CTL) responses has been described, the T cell receptor (TCR) diversity of virus-specific cells remains poorly defined. DESIGN AND METHODS: To address this issue, we applied a novel technique for subtractive analysis of the HIV-1-specific CTL repertoire, combining specific deletion of peptide-specific cells by 5-fluorouracil with TCR spectratyping to identify clonal breadth of CTL recognizing individual peptides. RESULTS: Comprehensive analysis of an infected individual reveals that nine identified HIV-1-specific responses are comprised of at least 38 distinct T-cell clones (ranging from two to 10 distinct clones per epitope). CONCLUSION: Given the potentially crucial role of T-cell receptor breadth for viral recognition and avoidance of escape, this subepitopic analysis of CTL may offer an important measure of cellular immunity for pathogenesis and vaccine studies.
OBJECTIVE: Although the epitopic breadth of HIV-1-specific CD8 T lymphocyte (CTL) responses has been described, the T cell receptor (TCR) diversity of virus-specific cells remains poorly defined. DESIGN AND METHODS: To address this issue, we applied a novel technique for subtractive analysis of the HIV-1-specific CTL repertoire, combining specific deletion of peptide-specific cells by 5-fluorouracil with TCR spectratyping to identify clonal breadth of CTL recognizing individual peptides. RESULTS: Comprehensive analysis of an infected individual reveals that nine identified HIV-1-specific responses are comprised of at least 38 distinct T-cell clones (ranging from two to 10 distinct clones per epitope). CONCLUSION: Given the potentially crucial role of T-cell receptor breadth for viral recognition and avoidance of escape, this subepitopic analysis of CTL may offer an important measure of cellular immunity for pathogenesis and vaccine studies.
Authors: Chungwon Chung; Wonhee Lee; John T Loffredo; Benjamin Burwitz; Thomas C Friedrich; Juan Pablo Giraldo Vela; Gnankang Napoe; Eva G Rakasz; Nancy A Wilson; David B Allison; David I Watkins Journal: J Virol Date: 2006-11-29 Impact factor: 5.103
Authors: John T Loffredo; Benjamin J Burwitz; Eva G Rakasz; Sean P Spencer; Jason J Stephany; Juan Pablo Giraldo Vela; Sarah R Martin; Jason Reed; Shari M Piaskowski; Jessica Furlott; Kim L Weisgrau; Denise S Rodrigues; Taeko Soma; Gnankang Napoé; Thomas C Friedrich; Nancy A Wilson; Esper G Kallas; David I Watkins Journal: J Virol Date: 2006-12-27 Impact factor: 5.103
Authors: Zoran Galić; Scott G Kitchen; Aparna Subramanian; Greg Bristol; Matthew D Marsden; Arumugam Balamurugan; Amelia Kacena; Otto Yang; Jerome A Zack Journal: Stem Cells Date: 2009-01 Impact factor: 6.277