PURPOSE: To assess vitreous metabolism by measuring C-propeptide levels of type II procollagen (pCOL-II-C) and hyaluronan levels in the vitreous and in the vitreous fluid after vitrectomy for macular hole. METHODS: We obtained 1-ml vitreous samples during vitrectomy from 34 patients (34 eyes) with a macular hole (age range 50-77 years, mean 64 years). After vitrectomy, we performed fluid-air exchange in six eyes because of unresolved macular holes and collected 4-ml fluid samples. Gel-filtration high-performance liquid chromatography (HPLC) was used to determine the molecular weight of pCOL-II-C in the samples. The pCOL-II-C level was measured by sandwich enzyme immunoassay and hyaluronan by sandwich binding protein assay. RESULTS: HPLC showed that pCOL-II-C in the vitreous samples corresponded to purified pCOL-II-C from cartilage. The vitreous pCOL-II-C level (4.7+/-0.3 ng/ml) was similar to reported synovial fluid levels. In six eyes that underwent fluid-air exchange, pCOL-II-C in the fluid samples remained at a level similar to that in the vitreous samples, while hyaluronan levels in the fluid samples were significantly lower than in the vitreous samples. CONCLUSIONS: The molecular weight and concentrations of pCOL-II-C in the vitreous are similar to those in joint fluid. In patients with a macular hole, type II procollagen may be secreted persistently into the vitreous cavity before and after vitrectomy.
PURPOSE: To assess vitreous metabolism by measuring C-propeptide levels of type II procollagen (pCOL-II-C) and hyaluronan levels in the vitreous and in the vitreous fluid after vitrectomy for macular hole. METHODS: We obtained 1-ml vitreous samples during vitrectomy from 34 patients (34 eyes) with a macular hole (age range 50-77 years, mean 64 years). After vitrectomy, we performed fluid-air exchange in six eyes because of unresolved macular holes and collected 4-ml fluid samples. Gel-filtration high-performance liquid chromatography (HPLC) was used to determine the molecular weight of pCOL-II-C in the samples. The pCOL-II-C level was measured by sandwich enzyme immunoassay and hyaluronan by sandwich binding protein assay. RESULTS: HPLC showed that pCOL-II-C in the vitreous samples corresponded to purified pCOL-II-C from cartilage. The vitreous pCOL-II-C level (4.7+/-0.3 ng/ml) was similar to reported synovial fluid levels. In six eyes that underwent fluid-air exchange, pCOL-II-C in the fluid samples remained at a level similar to that in the vitreous samples, while hyaluronan levels in the fluid samples were significantly lower than in the vitreous samples. CONCLUSIONS: The molecular weight and concentrations of pCOL-II-C in the vitreous are similar to those in joint fluid. In patients with a macular hole, type II procollagen may be secreted persistently into the vitreous cavity before and after vitrectomy.
Authors: T Ihanamäki; H Salminen; A M Säämänen; L J Pelliniemi; D J Hartmann; M Sandberg-Lall; E Vuorio Journal: Exp Eye Res Date: 2001-04 Impact factor: 3.467
Authors: D E Carey; M Alini; M Ionescu; J S Hyams; J C Rowe; L C Rosenberg; A R Poole Journal: Clin Exp Rheumatol Date: 1997 May-Jun Impact factor: 4.473
Authors: M Takanosu; T C Boyd; M Le Goff; S P Henry; Y Zhang; P N Bishop; R Mayne Journal: Invest Ophthalmol Vis Sci Date: 2001-09 Impact factor: 4.799
Authors: A J Richards; J Morgan; P W P Bearcroft; E Pickering; M J Owen; P Holmans; N Williams; C Tysoe; F M Pope; M P Snead; H Hughes Journal: J Med Genet Date: 2002-09 Impact factor: 6.318
Authors: F Nelson; L Dahlberg; S Laverty; A Reiner; I Pidoux; M Ionescu; G L Fraser; E Brooks; M Tanzer; L C Rosenberg; P Dieppe; A Robin Poole Journal: J Clin Invest Date: 1998-12-15 Impact factor: 14.808
Authors: Theodorus L Ponsioen; Marja J A van Luyn; Roelofje J van der Worp; Hendri H Pas; Johanna M M Hooymans; Leonoor I Los Journal: Mol Vis Date: 2008-03-26 Impact factor: 2.367