Literature DB >> 15897184

Mechanism and function of poleward flux in Xenopus extract meiotic spindles.

T J Mitchison1.   

Abstract

In Xenopus extract meiotic spindles, microtubules slide continuously towards their minus ends, a process called poleward flux. This article discusses recent progress in determining the mechanism of poleward flux, and its functions in spindle organization and generating force on chromosomes. Bipolar organization is required for flux and inhibition of the mitotic kinesin Eg5 inhibits flux, suggesting the sliding force for flux is generated by Eg5 pushing anti-parallel microtubules apart. An important function of flux in spindle organization may be to transport minus ends nucleated at chromatin towards the pole. By pulling microtubules through attachment sites at kinetochores, flux may generate poleward force on metaphase chromosomes.

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Year:  2005        PMID： 15897184      PMCID： PMC1569469          DOI： 10.1098/rstb.2004.1616

Source DB:  PubMed          Journal:  Philos Trans R Soc Lond B Biol Sci        ISSN： 0962-8436            Impact factor:   6.237


  25 in total

Review 1.  Microtubule motors in mitosis.

Authors:  D J Sharp; G C Rogers; J M Scholey
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Review 2.  The mitotic spindle: a self-made machine.

Authors:  E Karsenti; I Vernos
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3.  Recovery, visualization, and analysis of actin and tubulin polymer flow in live cells: a fluorescent speckle microscopy study.

Authors:  P Vallotton; A Ponti; C M Waterman-Storer; E D Salmon; G Danuser
Journal:  Biophys J       Date:  2003-08       Impact factor: 4.033

4.  An inner centromere protein that stimulates the microtubule depolymerizing activity of a KinI kinesin.

Authors:  Ryoma Ohi; Margaret L Coughlin; William S Lane; Timothy J Mitchison
Journal:  Dev Cell       Date:  2003-08       Impact factor: 12.270

Review 5.  The mitotic spindle and actin tails.

Authors:  Eric Karsenti; François Nédélec
Journal:  Biol Cell       Date:  2004-04       Impact factor: 4.458

Review 6.  Microtubule treadmills--possible molecular machinery.

Authors:  R L Margolis; L Wilson
Journal:  Nature       Date:  1981-10-29       Impact factor: 49.962

7.  Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles.

Authors:  T J Mitchison; P Maddox; J Gaetz; A Groen; M Shirasu; A Desai; E D Salmon; T M Kapoor
Journal:  Mol Biol Cell       Date:  2005-03-23       Impact factor: 4.138

8.  XRHAMM functions in ran-dependent microtubule nucleation and pole formation during anastral spindle assembly.

Authors:  Aaron C Groen; Lisa A Cameron; Margaret Coughlin; David T Miyamoto; Timothy J Mitchison; Ryoma Ohi
Journal:  Curr Biol       Date:  2004-10-26       Impact factor: 10.834

9.  Chromosome behavior after laser microirradiation of a single kinetochore in mitotic PtK2 cells.

Authors:  P A McNeill; M W Berns
Journal:  J Cell Biol       Date:  1981-03       Impact factor: 10.539

10.  Direct observation of microtubule dynamics at kinetochores in Xenopus extract spindles: implications for spindle mechanics.

Authors:  Paul Maddox; Aaron Straight; Peg Coughlin; Timothy J Mitchison; Edward D Salmon
Journal:  J Cell Biol       Date:  2003-08-04       Impact factor: 10.539
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  26 in total

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Authors:  Helder Maiato; Mariana Lince-Faria
Journal:  Cell Mol Life Sci       Date:  2010-03-21       Impact factor: 9.261

2.  Self-straining of actively crosslinked microtubule networks.

Authors:  Sebastian Fürthauer; Bezia Lemma; Peter J Foster; Stephanie C Ems-McClung; Che-Hang Yu; Claire E Walczak; Zvonimir Dogic; Daniel J Needleman; Michael J Shelley
Journal:  Nat Phys       Date:  2019-09-02       Impact factor: 20.034

Review 3.  Basic mechanism of eukaryotic chromosome segregation.

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Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2005-03-29       Impact factor: 6.237

4.  Measuring nanometer scale gradients in spindle microtubule dynamics using model convolution microscopy.

Authors:  Chad G Pearson; Melissa K Gardner; Leocadia V Paliulis; E D Salmon; David J Odde; Kerry Bloom
Journal:  Mol Biol Cell       Date:  2006-06-28       Impact factor: 4.138

5.  Tau-based fluorescent protein fusions to visualize microtubules.

Authors:  Paul Mooney; Taylor Sulerud; James F Pelletier; Matthew R Dilsaver; Miroslav Tomschik; Christoph Geisler; Jesse C Gatlin
Journal:  Cytoskeleton (Hoboken)       Date:  2017-05-22

6.  Poleward tubulin flux in spindles: regulation and function in mitotic cells.

Authors:  Daniel W Buster; Dong Zhang; David J Sharp
Journal:  Mol Biol Cell       Date:  2007-06-06       Impact factor: 4.138

7.  Op18 reveals the contribution of nonkinetochore microtubules to the dynamic organization of the vertebrate meiotic spindle.

Authors:  Benjamin R Houghtaling; Ge Yang; Alexandre Matov; Gaudenz Danuser; Tarun M Kapoor
Journal:  Proc Natl Acad Sci U S A       Date:  2009-08-19       Impact factor: 11.205

Review 8.  Do nuclear envelope and intranuclear proteins reorganize during mitosis to form an elastic, hydrogel-like spindle matrix?

Authors:  Kristen M Johansen; Arthur Forer; Changfu Yao; Jack Girton; Jørgen Johansen
Journal:  Chromosome Res       Date:  2011-04       Impact factor: 5.239

9.  Kinetochore-independent chromosome poleward movement during anaphase of meiosis II in mouse eggs.

Authors:  Manqi Deng; Juntao Gao; Praveen Suraneni; Rong Li
Journal:  PLoS One       Date:  2009-04-13       Impact factor: 3.240

10.  Synchronizing chromosome segregation by flux-dependent force equalization at kinetochores.

Authors:  Irina Matos; António J Pereira; Mariana Lince-Faria; Lisa A Cameron; Edward D Salmon; Helder Maiato
Journal:  J Cell Biol       Date:  2009-07-06       Impact factor: 10.539
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