Literature DB >> 15884903

Analytical ultracentrifugation for characterizing nanocrystals and their bioconjugates.

Michelle Calabretta1, Jennifer A Jamison, Joshua C Falkner, Yunping Liu, Benjamin D Yuhas, Kathleen S Matthews, Vicki L Colvin.   

Abstract

Analytical ultracentrifugation (AU) provides a general way to probe the polydispersity of nanoparticles and the formation of bioconjugates in solution. Unconjugated gold nanocrystals show sedimentation coefficient distributions that are in agreement with size distributions as measured by TEM. AU is sensitive to the size/shape changes elicited by conjugation, in this case to lactose repressor (LacI). AU data reveal saturating protein concentrations for conjugates that correspond to the measured stoichiometry of the complex under these conditions.

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Year:  2005        PMID： 15884903     DOI： 10.1021/nl047926f

Source DB:  PubMed          Journal:  Nano Lett        ISSN： 1530-6984            Impact factor:   11.189


  9 in total

1.  Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation.

Authors:  Patrick H Brown; Peter Schuck
Journal:  Biophys J       Date:  2006-03-24       Impact factor: 4.033

2.  A new adaptive grid-size algorithm for the simulation of sedimentation velocity profiles in analytical ultracentrifugation.

Authors:  Patrick H Brown; Peter Schuck
Journal:  Comput Phys Commun       Date:  2008-01-15       Impact factor: 4.390

3.  Synthesis, characterization, and direct intracellular imaging of ultrasmall and uniform glutathione-coated gold nanoparticles.

Authors:  Alioscka A Sousa; Jeffrey T Morgan; Patrick H Brown; April Adams; M P Suresh Jayasekara; Guofeng Zhang; Christopher J Ackerson; Michael J Kruhlak; Richard D Leapman
Journal:  Small       Date:  2012-04-20       Impact factor: 13.281

4.  In vitro and in vivo targeting of hollow gold nanoshells directed at epidermal growth factor receptor for photothermal ablation therapy.

Authors:  Marites P Melancon; Wei Lu; Zhi Yang; Rui Zhang; Zhi Cheng; Andrew M Elliot; Jason Stafford; Tammy Olson; Jin Z Zhang; Chun Li
Journal:  Mol Cancer Ther       Date:  2008-06       Impact factor: 6.261

5.  Sedimentation coefficient distributions of large particles.

Authors:  Peter Schuck
Journal:  Analyst       Date:  2016-05-19       Impact factor: 4.616

6.  Measuring the grafting density of nanoparticles in solution by analytical ultracentrifugation and total organic carbon analysis.

Authors:  Denise N Benoit; Huiguang Zhu; Michael H Lilierose; Raymond A Verm; Naushaba Ali; Adam N Morrison; John D Fortner; Carolina Avendano; Vicki L Colvin
Journal:  Anal Chem       Date:  2012-10-09       Impact factor: 6.986

7.  Biointeractions of ultrasmall glutathione-coated gold nanoparticles: effect of small size variations.

Authors:  Alioscka A Sousa; Sergio A Hassan; Luiza L Knittel; Andrea Balbo; Maria A Aronova; Patrick H Brown; Peter Schuck; Richard D Leapman
Journal:  Nanoscale       Date:  2016-03-28       Impact factor: 7.790

8.  In depth characterisation of the biomolecular coronas of polymer coated inorganic nanoparticles with differential centrifugal sedimentation.

Authors:  André Perez-Potti; Hender Lopez; Beatriz Pelaz; Abuelmagd Abdelmonem; Mahmoud G Soliman; Ingmar Schoen; Philip M Kelly; Kenneth A Dawson; Wolfgang J Parak; Zeljka Krpetic; Marco P Monopoli
Journal:  Sci Rep       Date:  2021-03-19       Impact factor: 4.379

9.  Characterization of size, anisotropy, and density heterogeneity of nanoparticles by sedimentation velocity.

Authors:  Borries Demeler; Tich-Lam Nguyen; Gary E Gorbet; Virgil Schirf; Emre H Brookes; Paul Mulvaney; Ala'a O El-Ballouli; Jun Pan; Osman M Bakr; Aysha K Demeler; Blanca I Hernandez Uribe; Nabraj Bhattarai; Robert L Whetten
Journal:  Anal Chem       Date:  2014-07-18       Impact factor: 6.986
  9 in total

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