Literature DB >> 15879035

Construction and expression of recombinant streptolysin-o and preevaluation of its use in immunoassays.

Blanca Velázquez1, Hugo Massaldi, Julio Battistoni, José A Chabalgoity.   

Abstract

Commercially available immunoassays for assessment of anti-streptolysin-O antibodies use native streptolysin-O obtained by a complex process. We prepared a biologically active recombinant streptolysin-O with higher yield and a simpler purification process. An enzyme-linked immunosorbent assay developed with this recombinant showed good correlation with a commercial test, suggesting that it could be suitable for immunoassays.

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Year:  2005        PMID： 15879035      PMCID： PMC1112085          DOI： 10.1128/CDLI.12.5.683-684.2005

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  10 in total

1.  Novel genomic rearrangement that affects expression of the Streptococcus pyogenes streptolysin O (slo) gene.

Authors:  Dragutin J Savic; Joseph J Ferretti
Journal:  J Bacteriol       Date:  2003-03       Impact factor: 3.490

Review 2.  Control of functional mRNA stability in bacteria: multiple mechanisms of nucleolytic and non-nucleolytic inactivation.

Authors:  C Petersen
Journal:  Mol Microbiol       Date:  1992-02       Impact factor: 3.501

3.  Evaluation of a new latex agglutination test for detection of streptolysin O antibodies.

Authors:  M A Gerber; L S Caparas; M F Randolph
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

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Authors:  S Bhakdi; M Roth; A Sziegoleit; J Tranum-Jensen
Journal:  Infect Immun       Date:  1984-11       Impact factor: 3.441

5.  Overlapping genes at the cheA locus of Escherichia coli.

Authors:  R A Smith; J S Parkinson
Journal:  Proc Natl Acad Sci U S A       Date:  1980-09       Impact factor: 11.205

6.  Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.

Authors:  D B Smith; K S Johnson
Journal:  Gene       Date:  1988-07-15       Impact factor: 3.688

7.  Cloning and expression in Escherichia coli of the streptolysin O determinant from Streptococcus pyogenes: characterization of the cloned streptolysin O determinant and demonstration of the absence of substantial homology with determinants of other thiol-activated toxins.

Authors:  M Kehoe; K N Timmis
Journal:  Infect Immun       Date:  1984-03       Impact factor: 3.441

8.  Translation of the sequence AGG-AGG yields 50% ribosomal frameshift.

Authors:  R A Spanjaard; J van Duin
Journal:  Proc Natl Acad Sci U S A       Date:  1988-11       Impact factor: 11.205

9.  Expression of tetanus toxin fragment C in E. coli: high level expression by removing rare codons.

Authors:  A J Makoff; M D Oxer; M A Romanos; N F Fairweather; S Ballantine
Journal:  Nucleic Acids Res       Date:  1989-12-25       Impact factor: 16.971

10.  Different forms of streptolysin O produced by Streptococcus pyogenes and by Escherichia coli expressing recombinant toxin: cleavage by streptococcal cysteine protease.

Authors:  M Pinkney; V Kapur; J Smith; U Weller; M Palmer; M Glanville; M Messner; J M Musser; S Bhakdi; M A Kehoe
Journal:  Infect Immun       Date:  1995-07       Impact factor: 3.441
  10 in total
  1 in total

1.  Non-Native Amino Acid Click Chemistry-Based Technology for Site-Specific Polysaccharide Conjugation to a Bacterial Protein Serving as Both Carrier and Vaccine Antigen.

Authors:  Neeraj Kapoor; Satoshi Uchiyama; Lucy Pill; Leslie Bautista; Angie Sedra; Lu Yin; Maritoni Regan; Ellen Chu; Taylor Rabara; Melissa Wong; Peter Davey; Jeff Fairman; Victor Nizet
Journal:  ACS Omega       Date:  2022-07-11
  1 in total

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