Literature DB >> 1587258

A gel retardation assay system for studying protein binding to simple repetitive DNA sequences.

W Mäueler1, M Muller, A C Köhne, J T Epplen.   

Abstract

Simple repetitive DNA sequences have been regarded as mere "junk" present in all eukaryotic genomes. In fact, mixed simple repeat (gt)n(ga)m sequences are present in major histocompatibility complex MHC-DRB genes for long evolutionary times, including such distant animals as artiodactyla and man. We describe herein an unsophisticated method which reveals that at least certain simple repetitive (gt)n(ga)m sequences bind nuclear proteins and show characteristics of a specific DNA-protein interaction via gel retardation.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1587258     DOI： 10.1002/elps.1150130103

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  5 in total

Review 1.  On the potential of simple repetitive DNA for fingerprinting in clinical, forensic, and evolutionary dynamic studies.

Authors:  J T Epplen; G Melmer; P Schmidt; L Roewer; J Hundrieser; C Epplen; J Buitkamp
Journal:  Clin Investig       Date:  1992-11

Review 2.  On genetic components in autoimmunity: a critical review based on evolutionarily oriented rationality.

Authors:  J T Epplen
Journal:  Hum Genet       Date:  1992-12       Impact factor: 4.132

3.  The paradox of MHC-DRB exon/intron evolution: alpha-helix and beta-sheet encoding regions diverge while hypervariable intronic simple repeats coevolve with beta-sheet codons.

Authors:  F W Schwaiger; E Weyers; C Epplen; J Brün; G Ruff; A Crawford; J T Epplen
Journal:  J Mol Evol       Date:  1993-09       Impact factor: 2.395

4.  Novel members and germline polymorphisms in the human T-cell receptor Vb6 family.

Authors:  M Gomolka; C Epplen; J Buitkamp; J T Epplen
Journal:  Immunogenetics       Date:  1993       Impact factor: 2.846

5.  Vb6 T-cell receptor elements in artiodactyls: conservation and germline polymorphisms.

Authors:  J Buitkamp; F W Schwaiger; J T Epplen
Journal:  Mamm Genome       Date:  1993-09       Impact factor: 2.957
  5 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.