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Literature DB >> 15872185

Human mitochondrial tRNAMet is exported to the cytoplasm and associates with the Argonaute 2 protein.

Elisavet Maniataki¹, Zissimos Mourelatos.

Abstract

Argonaute (Ago) proteins are the effector proteins of RNA interference (RNAi) and related silencing mechanisms that are mediated by small RNAs. Ago proteins bind directly to microRNAs (miRNAs) and to short interfering RNAs and are the core protein components of RNA induced silencing complexes (RISCs) and microRNPs (miRNPs). Here we report that an ~70-nt RNA associates specifically with immunopurified Ago2 expressed in human 293 cells. By directional cloning we identified this RNA as the mitochondrial tRNA(Met) (mt tRNA(Met)). Various exported (mt) tRNAs were detected in the cytosol of 293 cells, but Ago2 was found selectively bound to (mt) tRNA(Met). The association in the cytosol of exported (mt) tRNA(Met) with Ago2 complements genetic and microscopic data that link mitochondria with RNAi-related components and events.

Entities: Gene Species

Mesh：

Substances：

Year: 2005 PMID： 15872185 PMCID： PMC1370769 DOI： 10.1261/rna.2210805

Source DB: PubMed Journal: RNA ISSN： 1355-8382 Impact factor: 4.942

19 in total

Review 1. The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis.

Authors: Michelle A Carmell; Zhenyu Xuan; Michael Q Zhang; Gregory J Hannon
Journal: Genes Dev Date: 2002-11-01 Impact factor: 11.361

2. A genome-wide screen identifies 27 genes involved in transposon silencing in C. elegans.

Authors: Nadine L Vastenhouw; Sylvia E J Fischer; Valérie J P Robert; Karen L Thijssen; Andrew G Fraser; Ravi S Kamath; Julie Ahringer; Ronald H A Plasterk
Journal: Curr Biol Date: 2003-08-05 Impact factor: 10.834

Review 3. miRNAs on the move: miRNA biogenesis and the RNAi machinery.

Authors: Elizabeth P Murchison; Gregory J Hannon
Journal: Curr Opin Cell Biol Date: 2004-06 Impact factor: 8.382

4. Dissection of a natural RNA silencing process in the Drosophila melanogaster germ line.

Authors: Alexei A Aravin; Mikhail S Klenov; Vasilii V Vagin; Frédéric Bantignies; Giacomo Cavalli; Vladimir A Gvozdev
Journal: Mol Cell Biol Date: 2004-08 Impact factor: 4.272

5. Crystal structure of Argonaute and its implications for RISC slicer activity.

Authors: Ji-Joon Song; Stephanie K Smith; Gregory J Hannon; Leemor Joshua-Tor
Journal: Science Date: 2004-07-29 Impact factor: 47.728

6. miRNP:mRNA association in polyribosomes in a human neuronal cell line.

Authors: Peter T Nelson; Artemis G Hatzigeorgiou; Zissimos Mourelatos
Journal: RNA Date: 2004-03 Impact factor: 4.942

7. Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline.

Authors: A A Aravin; N M Naumova; A V Tulin; V V Vagin; Y M Rozovsky; V A Gvozdev
Journal: Curr Biol Date: 2001-07-10 Impact factor: 10.834

8. miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs.

Authors: Zissimos Mourelatos; Josée Dostie; Sergey Paushkin; Anup Sharma; Bernard Charroux; Linda Abel; Juri Rappsilber; Matthias Mann; Gideon Dreyfuss
Journal: Genes Dev Date: 2002-03-15 Impact factor: 11.361

9. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.

Authors: Gunter Meister; Markus Landthaler; Agnieszka Patkaniowska; Yair Dorsett; Grace Teng; Thomas Tuschl
Journal: Mol Cell Date: 2004-07-23 Impact factor: 17.970

10. Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors.

Authors: Noboru Doi; Shuhei Zenno; Ryu Ueda; Hiroko Ohki-Hamazaki; Kumiko Ui-Tei; Kaoru Saigo
Journal: Curr Biol Date: 2003-01-08 Impact factor: 10.834

51 in total

Review 1. Versatile TPR domains accommodate different modes of target protein recognition and function.

Authors: Rudi Kenneth Allan; Thomas Ratajczak
Journal: Cell Stress Chaperones Date: 2010-12-09 Impact factor: 3.667

Review 2. Desperately seeking microRNA targets.

Authors: Marshall Thomas; Judy Lieberman; Ashish Lal
Journal: Nat Struct Mol Biol Date: 2010-10 Impact factor: 15.369

3. Deep-sequencing of human Argonaute-associated small RNAs provides insight into miRNA sorting and reveals Argonaute association with RNA fragments of diverse origin.

Authors: Alexander Maxwell Burroughs; Yoshinari Ando; Michiel Jan Laurens de Hoon; Yasuhiro Tomaru; Harukazu Suzuki; Yoshihide Hayashizaki; Carsten Olivier Daub
Journal: RNA Biol Date: 2011-01-01 Impact factor: 4.652

Human mitochondrial tRNAMet is exported to the cytoplasm and associates with the Argonaute 2 protein.

Review 1. The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis.

2. A genome-wide screen identifies 27 genes involved in transposon silencing in C. elegans.

Review 3. miRNAs on the move: miRNA biogenesis and the RNAi machinery.

4. Dissection of a natural RNA silencing process in the Drosophila melanogaster germ line.

5. Crystal structure of Argonaute and its implications for RISC slicer activity.

6. miRNP:mRNA association in polyribosomes in a human neuronal cell line.

7. Double-stranded RNA-mediated silencing of genomic tandem repeats and transposable elements in the D. melanogaster germline.

8. miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs.

9. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs.

10. Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors.

Review 1. Versatile TPR domains accommodate different modes of target protein recognition and function.

Review 2. Desperately seeking microRNA targets.

3. Deep-sequencing of human Argonaute-associated small RNAs provides insight into miRNA sorting and reveals Argonaute association with RNA fragments of diverse origin.

4. The ins and outs of tRNA transport.

5. Site-specific crosslinking of human microRNPs to RNA targets.

Review 6. Regulation of mammalian microRNA processing and function by cellular signaling and subcellular localization.

7. Mitochondria as novel players of the cellular RNA interference.

8. Argonaute 2 Binds Directly to tRNA Genes and Promotes Gene Repression in cis.

9. Biochemical and genetic evidence for a role of IGHMBP2 in the translational machinery.

10. Ago2 immunoprecipitation identifies predicted microRNAs in human embryonic stem cells and neural precursors.