BACKGROUND: Variants in the CD14 gene (CD14) are hypothesized to be associated with atopic disorders. However, most studies have only investigated one polymorphism in this gene. OBJECTIVE: We sought to study the association of 5 single nucleotide polymorphisms (SNPs) in the 5' flanking region of CD14 with eczema and serum IgE levels in young children. METHODS: We genotyped 5 SNPs in an approximately 6.5-kb region in the 5' region of CD14 in 344 2-year-old white children from 2 birth cohorts in the northeastern United States. We examined the relation of both single SNPs and haplotypes in CD14 with the atopic outcomes. RESULTS: Two SNPs were significantly associated with eczema. In dominant models adjusted for potential confounders, SNP rs2569193 was associated with significantly decreased risk for eczema (odds ratio [OR] for CT/TT vs CC, 0.5; 95% CI, 0.3-0.8), whereas SNP rs2569190 (also reported as the C-159T) was associated with significantly increased risk for eczema (OR for CT/TT vs CC, 2.3; 95% CI, 1.4-3.8). The CT/TT genotypes of SNP rs2569190 also had higher geometric means of serum IgE than the CC genotype (24.6 vs 15 IU/mL, P = .025). Haplotype analyses provided results similar to those of the single SNP analyses. CONCLUSIONS: Our results contradict previous reports that have found a protective effect of the T allele of SNP rs2569190 (C-159T) against atopic disorders. Nevertheless, these results confirm the importance of polymorphisms in CD14 in the development of atopy, and future studies of this gene region will need to account for linkage disequilibrium and environmental exposures unique to the study population.
BACKGROUND: Variants in the CD14 gene (CD14) are hypothesized to be associated with atopic disorders. However, most studies have only investigated one polymorphism in this gene. OBJECTIVE: We sought to study the association of 5 single nucleotide polymorphisms (SNPs) in the 5' flanking region of CD14 with eczema and serum IgE levels in young children. METHODS: We genotyped 5 SNPs in an approximately 6.5-kb region in the 5' region of CD14 in 344 2-year-old white children from 2 birth cohorts in the northeastern United States. We examined the relation of both single SNPs and haplotypes in CD14 with the atopic outcomes. RESULTS: Two SNPs were significantly associated with eczema. In dominant models adjusted for potential confounders, SNP rs2569193 was associated with significantly decreased risk for eczema (odds ratio [OR] for CT/TT vs CC, 0.5; 95% CI, 0.3-0.8), whereas SNP rs2569190 (also reported as the C-159T) was associated with significantly increased risk for eczema (OR for CT/TT vs CC, 2.3; 95% CI, 1.4-3.8). The CT/TT genotypes of SNP rs2569190 also had higher geometric means of serum IgE than the CC genotype (24.6 vs 15 IU/mL, P = .025). Haplotype analyses provided results similar to those of the single SNP analyses. CONCLUSIONS: Our results contradict previous reports that have found a protective effect of the T allele of SNP rs2569190 (C-159T) against atopic disorders. Nevertheless, these results confirm the importance of polymorphisms in CD14 in the development of atopy, and future studies of this gene region will need to account for linkage disequilibrium and environmental exposures unique to the study population.
Authors: Marie Klumplerova; Leona Vychodilova; Olga Bobrova; Michaela Cvanova; Jan Futas; Eva Janova; Mirko Vyskocil; Irena Vrtkova; Lenka Putnova; Ladislav Dusek; Eliane Marti; Petr Horin Journal: Mol Biol Rep Date: 2012-12-30 Impact factor: 2.316
Authors: Sunita Sharma; Audrey Poon; Blanca E Himes; Jessica Lasky-Su; Joanne E Sordillo; Kathleen Belanger; Donald K Milton; Michael B Bracken; Elizabeth W Triche; Brian P Leaderer; Diane R Gold; Augusto A Litonjua Journal: Pediatr Allergy Immunol Date: 2011-12-23 Impact factor: 6.377
Authors: L Keoki Williams; Jennifer Oliver; Edward L Peterson; Kevin R Bobbitt; Michael J McCabe; Derek Smolarek; Suzanne L Havstad; Ganesa Wegienka; Esteban G Burchard; Dennis R Ownby; Christine C Johnson Journal: Ann Allergy Asthma Immunol Date: 2008-02 Impact factor: 6.347
Authors: Joanne E Sordillo; Sunita Sharma; Audrey Poon; Jessica Lasky-Su; Kathleen Belanger; Donald K Milton; Michael B Bracken; Elizabeth W Triche; Brian P Leaderer; Diane R Gold; Augusto A Litonjua Journal: BMC Med Genet Date: 2011-12-08 Impact factor: 2.103
Authors: Tobias T Schimming; Qumar Parwez; Elisabeth Petrasch-Parwez; Michael Nothnagel; Joerg T Epplen; Sabine Hoffjan Journal: BMC Dermatol Date: 2007-03-16