| Literature DB >> 15848073 |
William Ying Khee Hwang1, Juan Carlos Almagro, Timothy N Buss, Philip Tan, Jefferson Foote.
Abstract
We report a new method of humanizing antibodies by complementarity determining region (CDR) grafting. Our method differs from others in that we choose human framework sequences from the set of human germline genes based on the structural similarity of the human CDRs to those of the mouse antibody to be humanized. The structural similarity is evaluated by scoring residue-to-residue homology of the mouse CDRs to human candidates with the same Chothia canonical structures. The method is illustrated with the humanization of the anti-lysozyme antibody D1.3.Entities:
Mesh：
Substances：
Year: 2005 PMID： 15848073 DOI： 10.1016/j.ymeth.2005.01.004
Source DB: PubMed Journal: Methods ISSN： 1046-2023 Impact factor: 3.608