Literature DB >> 1584785

Methods for the large-scale synthesis of psoralen furan-side monoadducts and diadducts.

H P Spielmann1, S S Sastry, J E Hearst.   

Abstract

We report methods for the preparation of multimicromole quantities of extremely pure uniquely photo-adducted psoralen-DNA furan-side monoadducts and diadducts (cross-links). The methods use high-intensity krypton and argon ion lasers in the photoreactions and HPLC methods to purify the required oligonucleotides containing the photoadducts. With these methods we have synthesized 2-3 mumol of 8-mer psoralen furan-side monoadduct and diadduct. These methods allow one to generate large amounts of psoralenated DNA oligonucleotides and facilitate their study by NMR and x-ray crystallography.

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Year:  1992        PMID： 1584785      PMCID： PMC49113          DOI： 10.1073/pnas.89.10.4514

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  30 in total

1.  Sequence-specific photo-induced cross-linking of the two strands of double-helical DNA by a psoralen covalently linked to a triple helix-forming oligonucleotide.

Authors:  M Takasugi; A Guendouz; M Chassignol; J L Decout; J Lhomme; N T Thuong; C Hélène
Journal:  Proc Natl Acad Sci U S A       Date:  1991-07-01       Impact factor: 11.205

2.  Studies on the interaction of T7 RNA polymerase with a DNA template containing a site-specifically placed psoralen cross-link. II. Stability and some properties of elongation complexes.

Authors:  S S Sastry; J E Hearst
Journal:  J Mol Biol       Date:  1991-10-20       Impact factor: 5.469

3.  The use of psoralen-modified DNA to probe the mechanism of enhancer action.

Authors:  A J Courey; S E Plon; J C Wang
Journal:  Cell       Date:  1986-05-23       Impact factor: 41.582

4.  Base-catalyzed reversal of a psoralen-DNA cross-link.

Authors:  Y B Shi; H P Spielmann; J E Hearst
Journal:  Biochemistry       Date:  1988-07-12       Impact factor: 3.162

5.  Structure of a psoralen cross-linked DNA in solution by nuclear magnetic resonance.

Authors:  M T Tomic; D E Wemmer; S H Kim
Journal:  Science       Date:  1987-12-18       Impact factor: 47.728

6.  Alkali reversal of psoralen cross-link for the targeted delivery of psoralen monoadduct lesion.

Authors:  A T Yeung; W J Dinehart; B K Jones
Journal:  Biochemistry       Date:  1988-08-23       Impact factor: 3.162

7.  Photochemical decontamination of blood components containing hepatitis B and non-A, non-B virus.

Authors:  H J Alter; R P Creagan; P A Morel; G P Wiesehahn; B P Dorman; L Corash; G C Smith; H Popper; J W Eichberg
Journal:  Lancet       Date:  1988 Dec 24-31       Impact factor: 79.321

8.  RecA-dependent incision of psoralen-crosslinked DNA by (A)BC excinuclease.

Authors:  S Cheng; A Sancar; J E Hearst
Journal:  Nucleic Acids Res       Date:  1991-02-11       Impact factor: 16.971

9.  Triple-helical DNA pairing intermediates formed by recA protein.

Authors:  S W Umlauf; M M Cox; R B Inman
Journal:  J Biol Chem       Date:  1990-10-05       Impact factor: 5.157

10.  Use of 8-methoxypsoralen and long-wavelength ultraviolet radiation for decontamination of platelet concentrates.

Authors:  L Lin; G P Wiesehahn; P A Morel; L Corash
Journal:  Blood       Date:  1989-07       Impact factor: 22.113
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  7 in total

1.  DNA interstrand cross-links induce futile repair synthesis in mammalian cell extracts.

Authors:  D Mu; T Bessho; L V Nechev; D J Chen; T M Harris; J E Hearst; A Sancar
Journal:  Mol Cell Biol       Date:  2000-04       Impact factor: 4.272

Review 2.  Using synthetic DNA interstrand crosslinks to elucidate repair pathways and identify new therapeutic targets for cancer chemotherapy.

Authors:  Angelo Guainazzi; Orlando D Schärer
Journal:  Cell Mol Life Sci       Date:  2010-08-21       Impact factor: 9.261

Review 3.  Formation and repair of interstrand cross-links in DNA.

Authors:  David M Noll; Tracey McGregor Mason; Paul S Miller
Journal:  Chem Rev       Date:  2006-02       Impact factor: 60.622

4.  Single Molecule Analysis of Laser Localized Psoralen Adducts.

Authors:  Jing Huang; Himabindu Gali; Julia Gichimu; Marina A Bellani; Durga Pokharel; Manikandan Paramasivam; Michael M Seidman
Journal:  J Vis Exp       Date:  2017-04-20       Impact factor: 1.355

5.  DNA structural reorganization upon conversion of a psoralen furan-side monoadduct to an interstrand cross-link: implications for DNA repair.

Authors:  H P Spielmann; T J Dwyer; S S Sastry; J E Hearst; D E Wemmer
Journal:  Proc Natl Acad Sci U S A       Date:  1995-03-14       Impact factor: 11.205

6.  Quantitative analysis of DNA interstrand cross-links and monoadducts formed in human cells induced by psoralens and UVA irradiation.

Authors:  Congfang Lai; Huachuan Cao; John E Hearst; Laurence Corash; Hai Luo; Yinsheng Wang
Journal:  Anal Chem       Date:  2008-10-24       Impact factor: 6.986

7.  Psoralen Derivatives with Enhanced Potency.

Authors:  Alexandru D Buhimschi; David M Gooden; Hongwu Jing; Diane R Fels; Katherine S Hansen; Wayne F Beyer; Mark W Dewhirst; Harold Walder; Francis P Gasparro
Journal:  Photochem Photobiol       Date:  2020-06-24       Impact factor: 3.421
  7 in total

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