Literature DB >> 15834791

Purification and characterization of an alkaline cellulase from a newly isolated alkalophilic Bacillus sp. HSH-810.

Ji-Yeon Kim1, Sung-Ho Hur, Jeong-Hwa Hong.   

Abstract

An alkaline cellulase from Bacillus sp. HSH-810 was purified 8.7-fold with a 30% yield and a specific activity of 71 U mg(-1) protein. It was optimally active at pH 10 and 50 degrees C and was stable from pH 6 to 10 with more than 60% activity remaining after heating at 60 degrees C for 60 min. The molecular mass of cellulase was 80 kDa. It was inhibited by 50% by Fe3+ (1 mM) and Mn2+ (0.1 mM) but was relatively insensitive to Hg2+ and Pb2+ at 1 mM.

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Year:  2005        PMID： 15834791     DOI： 10.1007/s10529-005-0685-5

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  10 in total

1.  Assessment of the biomass hydrolysis potential in bacterial isolates from a volcanic environment: biosynthesis of the corresponding activities.

Authors:  Panagiota M Stathopoulou; Anastasia P Galanopoulou; George E Anasontzis; Amalia D Karagouni; Dimitris G Hatzinikolaou
Journal:  World J Microbiol Biotechnol       Date:  2012-06-20       Impact factor: 3.312

2.  Purification and characterization of an acidothermophilic cellulase enzyme produced by Bacillus subtilis strain LFS3.

Authors:  Rekha Rawat; Lakshmi Tewari
Journal:  Extremophiles       Date:  2012-05-18       Impact factor: 2.395

3.  Bioprocess development for enhanced endoglucanase production by newly isolated bacteria, purification, characterization and in-vitro efficacy as anti-biofilm of Pseudomonas aeruginosa.

Authors:  Atef M Ibrahim; Ragaa A Hamouda; Noura El-Ahmady El-Naggar; Fatma M Al-Shakankery
Journal:  Sci Rep       Date:  2021-05-07       Impact factor: 4.379

4.  Isolation, production, purification and characterization of an organic-solvent-thermostable alkalophilic cellulase from Bacillus vallismortis RG-07.

Authors:  Rajeeva Gaur; Soni Tiwari
Journal:  BMC Biotechnol       Date:  2015-03-19       Impact factor: 2.563

5.  Purification and biochemical properties of a thermostable, haloalkaline cellulase from Bacillus licheniformis AMF-07 and its application for hydrolysis of different cellulosic substrates to bioethanol production.

Authors:  Fatemeh Azadian; Arastoo Badoei-Dalfard; Abdolhamid Namaki-Shoushtari; Mehdi Hassanshahian
Journal:  Mol Biol Res Commun       Date:  2016-09

6.  Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses.

Authors:  Farjana Islam; Narayan Roy
Journal:  BMC Res Notes       Date:  2018-07-04

7.  Identification and characterization of an Endo-glucanase secreted from cellulolytic Escherichia coli ZH-4.

Authors:  Jian Pang; Junshu Wang; Zhanying Liu; Qiancheng Zhang; Qingsheng Qi
Journal:  BMC Biotechnol       Date:  2019-08-27       Impact factor: 2.563

8.  Production, purification and characterization of a novel thermotolerant endoglucanase (CMCase) from Bacillus strain isolated from cow dung.

Authors:  Sangrila Sadhu; Pradipta Saha; Sukanta K Sen; Shanmugam Mayilraj; Tushar Kanti Maiti
Journal:  Springerplus       Date:  2013-01-12

9.  Expression, purification and characterization of an endoglucanase from Serratia proteamaculans CDBB-1961, isolated from the gut of Dendroctonus adjunctus (Coleoptera: Scolytinae).

Authors:  Claudia Cano-Ramírez; Alejandro Santiago-Hernández; Flor Nohemí Rivera-Orduña; Yolanda García-Huante; Gerardo Zúñiga; María Eugenia Hidalgo-Lara
Journal:  AMB Express       Date:  2016-08-31       Impact factor: 3.298

10.  Production, purification and characterization of an acid/alkali and thermo tolerant cellulase from Schizophyllum commune NAIMCC-F-03379 and its application in hydrolysis of lignocellulosic wastes.

Authors:  Bikash Kumar; Nisha Bhardwaj; Ansar Alam; Komal Agrawal; Himanshu Prasad; Pradeep Verma
Journal:  AMB Express       Date:  2018-10-17       Impact factor: 3.298
  10 in total

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