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Literature DB >> 15810830

Nucleobase participation in ribozyme catalysis.

Zheng-Yun Zhao¹, Aileen McLeod, Shinya Harusawa, Lisa Araki, Maho Yamaguchi, Takushi Kurihara, David M J Lilley.

Abstract

We constructed a modified form of the VS ribozyme containing an imidazole ring in place of adenine at position 756. The novel ribozyme is active in both cleavage and ligation reactions. The reaction is efficient, although relatively slow. The results are consistent with a role for nucleobase catalysis in the catalytic mechanism of this ribozyme.

Entities: Chemical

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Year: 2005 PMID： 15810830 DOI： 10.1021/ja0502775

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

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Cited

19 in total

1. Catalytic importance of a protonated adenosine in the hairpin ribozyme active site.

Authors: Ian T Suydam; Stephen D Levandoski; Scott A Strobel
Journal: Biochemistry Date: 2010-05-04 Impact factor: 3.162

2. Evidence for proton transfer in the rate-limiting step of a fast-cleaving Varkud satellite ribozyme.

Authors: M Duane Smith; Richard A Collins
Journal: Proc Natl Acad Sci U S A Date: 2007-03-26 Impact factor: 11.205

3. Fluorine substituted adenosines as probes of nucleobase protonation in functional RNAs.

Authors: Ian T Suydam; Scott A Strobel
Journal: J Am Chem Soc Date: 2008-09-20 Impact factor: 15.419

4. Nucleobase-mediated general acid-base catalysis in the Varkud satellite ribozyme.

Authors: Timothy J Wilson; Nan-Sheng Li; Jun Lu; John K Frederiksen; Joseph A Piccirilli; David M J Lilley
Journal: Proc Natl Acad Sci U S A Date: 2010-06-14 Impact factor: 11.205

5. Do the hairpin and VS ribozymes share a common catalytic mechanism based on general acid-base catalysis? A critical assessment of available experimental data.

Authors: Timothy J Wilson; David M J Lilley
Journal: RNA Date: 2010-12-20 Impact factor: 4.942

Nucleobase participation in ribozyme catalysis.

1. Catalytic importance of a protonated adenosine in the hairpin ribozyme active site.

2. Evidence for proton transfer in the rate-limiting step of a fast-cleaving Varkud satellite ribozyme.

3. Fluorine substituted adenosines as probes of nucleobase protonation in functional RNAs.

4. Nucleobase-mediated general acid-base catalysis in the Varkud satellite ribozyme.

5. Do the hairpin and VS ribozymes share a common catalytic mechanism based on general acid-base catalysis? A critical assessment of available experimental data.

6. Nucleobase catalysis in the hairpin ribozyme.

Review 7. DNA as a versatile chemical component for catalysis, encoding, and stereocontrol.

8. The complete VS ribozyme in solution studied by small-angle X-ray scattering.

9. Mechanisms of RNA catalysis.

10. A guanine nucleobase important for catalysis by the VS ribozyme.