[Content determination of loganic acid and gentiopicroside in Tebitan herbal medicines Gentiana macrophylla and G. straminea by HPLC].

A quantitative method to determine loganic acid and gentiopicroside in Gentiana macrophylla Pall. and Gentiana straminea Maxim. by RP-HPLC was established in this paper. The two compounds were base-isolated on the coloumn of Zorbax SB-C18 (250 mm x 4.6 mm i.d., 5 microm) which eluted with MeOH and H2O (0.04% H3PO4). The methol ratio increased from 18% to 28% in 15 min with detective wave at 254 nm and coloumn temperature at 30 degree C, flow rate at 1 ml/min. A wide range of linearity and the good linear relationships were achieved on above condition. The contents of the two compounds in different parts of the two plants were also determined and compared in this paper.