| Literature DB >> 15797623 |
Masahiko Kuroda1, Kosuke Oikawa, Keiichi Yoshida, Aya Takeuchi, Masaru Takeuchi, Masahiko Usui, Akihiro Umezawa, Kiyoshi Mukai.
Abstract
hWAPL is a human oncogene associated with uterine cervical cancer. Here, we demonstrate that hWAPL transcription is induced by 3-methylcholanthrene (3-MC) in the cervical carcinoma-derived cell line SiHa. hWAPL transcription was analyzed with evaluation of the mRNA and heterogeneous nuclear RNA (hnRNA) levels by quantitative real time PCR analysis. Flow cytometric analysis suggested that the alteration of hWAPL mRNA levels is independent of cell cycle profile. We also found that DMSO and some components of FBS affect hWAPL transcription. Interestingly, when the aryl hydrocarbon receptor (AhR) function was inhibited by alpha-naphthoflavone (ANF), the induction of hWAPL transcription by 3-MC was greater than that in AhR-functioning normal cells. These observations suggest that there are complex mechanisms regulating the transcription of hWAPL. Furthermore, mRNA level of a mouse homolog of hWAPL in mouse uterus was induced by 3-MC injection into the abdominal cavity. Thus, some effects from 3-MC exposure on uterus may be mediated by the unscheduled overexpression of hWAPL.Entities:
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Year: 2005 PMID： 15797623 DOI： 10.1016/j.canlet.2004.08.006
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679