| Literature DB >> 15797213 |
Wen Liu1, Koichi Nonaka, Liping Nie, Jian Zhang, Steven D Christenson, Juyun Bae, Steven G Van Lanen, Emmanuel Zazopoulos, Chris M Farnet, Catherine F Yang, Ben Shen.
Abstract
The biosynthetic gene cluster for the enediyne antitumor antibiotic neocarzinostatin (NCS) was localized to 130 kb continuous DNA from Streptomyces carzinostaticus ATCC15944 and confirmed by gene inactivation. DNA sequence analysis of 92 kb of the cloned region revealed 68 open reading frames (ORFs), 47 of which were determined to constitute the NCS cluster. Sequence analysis of the genes within the NCS cluster suggested dNDP-D-mannose as a precursor for the deoxy aminosugar, revealed two distinct type I polyketide synthases (PKSs), and supported a convergent model for NCS chromophore biosynthesis from the deoxy aminosugar, naphthoic acid, and enediyne core building blocks. These findings shed light into deoxysugar biosynthesis, further support the iterative type I PKS paradigm for enediyne core biosynthesis, and unveil a mechanism for microbial polycyclic aromatic polyketide biosynthesis by an iterative type I PKS.Entities:
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Year: 2005 PMID： 15797213 DOI： 10.1016/j.chembiol.2004.12.013
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521