Literature DB >> 1579454

A single amino acid change restores DNA cytosine methyltransferase activity in a cloned chlorella virus pseudogene.

Y Zhang1, M Nelson, J L Van Etten.   

Abstract

The chlorella virus PBCV-1 contains an open reading frame, named P17-ORF4, which differs by eight amino acids from a DNA cytosine methyltransferase, M.CviJI, encoded by a different chlorella virus IL-3A. Whereas IL-3A expresses M.CviJI, which methylates the central cytosine in (A/G)GC(T/C/G) sequences, P17-ORF4 is non-functional. Gene fusions between P17-ORF4 and M.CviJI and site-directed point mutations revealed that changing Gln188 to Lys188 abolishes M.CviJI methyltransferase activity. Conversely, changing Lys188 in P17-ORF4 to Gln188 results in M.CviJI activity. The other altered seven amino acids do not appear to affect M.CviJI activity.

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Year:  1992        PMID： 1579454      PMCID： PMC312249          DOI： 10.1093/nar/20.7.1637

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

1.  'Pseudo' domains in phage-encoded DNA methyltransferases.

Authors:  C Lange; A Jugel; J Walter; M Noyer-Weidner; T A Trautner
Journal:  Nature       Date:  1991-08-15       Impact factor: 49.962

Review 2.  Viruses and viruslike particles of eukaryotic algae.

Authors:  J L Van Etten; L C Lane; R H Meints
Journal:  Microbiol Rev       Date:  1991-12

3.  Chlorella viruses isolated in China.

Authors:  Y P Zhang; D E Burbank; J L Van Etten
Journal:  Appl Environ Microbiol       Date:  1988-09       Impact factor: 4.792

4.  Lytic viruses infecting a Chlorella-like alga.

Authors:  J L Van Etten; D E Burbank; A M Schuster; R H Meints
Journal:  Virology       Date:  1985-01-15       Impact factor: 3.616

5.  Characterization of viruses infecting a eukaryotic Chlorella-like green alga.

Authors:  A M Schuster; D E Burbank; B Meister; M P Skrdla; R H Meints; S Hattman; D Swinton; J L Van Etten
Journal:  Virology       Date:  1986-04-15       Impact factor: 3.616

6.  5-Methylcytosine replacing cytosine in the deoxyribonucleic acid of a bacteriophage for Xanthomonas oryzae.

Authors:  T T Kuo; T C Huang; M H Teng
Journal:  J Mol Biol       Date:  1968-07-14       Impact factor: 5.469

7.  IL-3A virus infection of a Chlorella-like green alga induces a DNA restriction endonuclease with novel sequence specificity.

Authors:  Y N Xia; D E Burbank; L Uher; D Rabussay; J L Van Etten
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

8.  Restriction endonuclease activity induced by PBCV-1 virus infection of a Chlorella-like green alga.

Authors:  Y N Xia; D E Burbank; L Uher; D Rabussay; J L Van Etten
Journal:  Mol Cell Biol       Date:  1986-05       Impact factor: 4.272

9.  DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

Authors:  S Tabor; C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1987-07       Impact factor: 11.205

10.  The termini of the chlorella virus PBCV-1 genome are identical 2.2-kbp inverted repeats.

Authors:  P Strasser; Y P Zhang; J Rohozinski; J L Van Etten
Journal:  Virology       Date:  1991-02       Impact factor: 3.616
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  5 in total

1.  Sequencing two DNA templates in five channels by digital compression.

Authors:  M Nelson; Y Zhang; D L Steffens; R Grabherr; J L Van Etten
Journal:  Proc Natl Acad Sci U S A       Date:  1993-03-01       Impact factor: 11.205

2.  REBASE--restriction enzymes and methylases.

Authors:  R J Roberts; D Macelis
Journal:  Nucleic Acids Res       Date:  1993-07-01       Impact factor: 16.971

Review 3.  The DNA (cytosine-5) methyltransferases.

Authors:  S Kumar; X Cheng; S Klimasauskas; S Mi; J Posfai; R J Roberts; G G Wilson
Journal:  Nucleic Acids Res       Date:  1994-01-11       Impact factor: 16.971

4.  Cloning, characterization and expression of the gene coding for a cytosine-5-DNA methyltransferase recognizing GpC.

Authors:  M Xu; M P Kladde; J L Van Etten; R T Simpson
Journal:  Nucleic Acids Res       Date:  1998-09-01       Impact factor: 16.971

5.  Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system.

Authors:  Y Zhang; M Nelson; J W Nietfeldt; D E Burbank; J L Van Etten
Journal:  Nucleic Acids Res       Date:  1992-10-25       Impact factor: 16.971
  5 in total

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