Jamshid Kohanteb1, Sadreddin Ardehali. 1. Department of Microbiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. kohanj@sums.ac.ir
Abstract
OBJECTIVE: To compare and evaluate the application of indirect fluorescent antibody (IFA) and counterimmunoelectrophoresis (CIEP) for laboratory identification of visceral leishmaniasis. MATERIALS AND METHODS: Serum samples from patients with malaria (Plasmodium vivax, n = 86; Plasmodium falciparum, n = 38), brucellosis (n = 26), tuberculosis (n = 31) and typhoid fever (n = 35) were examined for the presence of antibody to Leishmaniainfantum antigen using IFA and CIEP tests. RESULTS: Using IFA, false-positive results were malaria (P. vivax 19.8%, P. falciparum 13.2%), tuberculosis (6.4%), brucellosis (3.8%), and typhoid fever (2.8%). Using CIEP, a lower percentage of false-positives was observed only among malaria patients (P. vivax 2.3%, P. falciparum 2.6%). Serum samples from patients with other infectious diseases were negative in the CIEP test. CONCLUSION: Based on the results of this study, the CIEP technique is recommended for immunodiagnosis of visceral leishmaniasis, especially in regions where malaria, brucellosis and tuberculosis are prevalent. 2005 S. Karger AG, Basel.
OBJECTIVE: To compare and evaluate the application of indirect fluorescent antibody (IFA) and counterimmunoelectrophoresis (CIEP) for laboratory identification of visceral leishmaniasis. MATERIALS AND METHODS: Serum samples from patients with malaria (Plasmodium vivax, n = 86; Plasmodium falciparum, n = 38), brucellosis (n = 26), tuberculosis (n = 31) and typhoid fever (n = 35) were examined for the presence of antibody to Leishmaniainfantum antigen using IFA and CIEP tests. RESULTS: Using IFA, false-positive results were malaria (P. vivax 19.8%, P. falciparum 13.2%), tuberculosis (6.4%), brucellosis (3.8%), and typhoid fever (2.8%). Using CIEP, a lower percentage of false-positives was observed only among malariapatients (P. vivax 2.3%, P. falciparum 2.6%). Serum samples from patients with other infectious diseases were negative in the CIEP test. CONCLUSION: Based on the results of this study, the CIEP technique is recommended for immunodiagnosis of visceral leishmaniasis, especially in regions where malaria, brucellosis and tuberculosis are prevalent. 2005 S. Karger AG, Basel.
Authors: David Pace; Thomas N Williams; Alicja Grochowska; Alexandra Betts; Simon Attard-Montalto; Michael J Boffa; Cecil Vella Journal: Travel Med Infect Dis Date: 2011-01-05 Impact factor: 6.211
Authors: Mathieu Bangert; María D Flores-Chávez; Ivonne P Llanes-Acevedo; Carolina Arcones; Carmen Chicharro; Emilia García; Sheila Ortega; Javier Nieto; Israel Cruz Journal: PLoS Negl Trop Dis Date: 2018-03-01
Authors: Anna Raquel Ribeiro Dos Santos; Ângela Vieira Serufo; Maria Marta Figueiredo; Lara Carvalho Godoi; Jéssica Gardone Vitório; Andreza Pain Marcelino; Daniel Moreira de Avelar; Fernandes Tenório Gomes Rodrigues; George Luiz Lins Machado-Coelho; Fernanda Alvarenga Cardoso Medeiros; Selma Maria Bezerra Jerônimo; Edward José de Oliveira; Frederico Crepaldi Nascimento; Santuza Maria Ribeiro Teixeira; Ricardo Tostes Gazzinelli; Ronaldo Alves Pinto Nagem; Ana Paula Fernandes Journal: Mem Inst Oswaldo Cruz Date: 2019-02-04 Impact factor: 2.743
Authors: Gláucia Cota; Julia Walochnik; Astrid Christine Erber; Peter Julian Sandler; Daniel Moreira de Avelar; Ines Swoboda Journal: Parasit Vectors Date: 2022-01-24 Impact factor: 3.876