Literature DB >> 15782961

Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography.

Yoichiro Ito1.   

Abstract

This paper aims to be an aid to those chemists who are interested in utilizing high-speed counter-current chromatography (HSCCC), which is free of irreversible adsorption and offers high resolution comparable to column chromatography. It explains the selection of HSCCC conditions step by step including the selection of two-phase solvent systems, determination of partition coefficient (K) of analytes, preparation of two-phase solvent system and sample solution, selection of elution mode, flow rate, rotation speed, and on-line monitoring of the eluate. The paper covers both standard HSCCC and pH-zone-refining CCC techniques. Technical terms (italic) unfamiliar to the beginner are comprehensively explained in Glossary. Various examples of two-phase solvent systems used in HSCCC are listed in Appendices A and B. The commercial sources of HSCCC and other CCC instruments are described in detail in the study edited by Berthod [A. Berthod (Ed.), Counter-current Chromatography, Elsevier, Amsterdam, 2003].

Mesh：

Year:  2005        PMID： 15782961     DOI： 10.1016/j.chroma.2004.12.044

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  160 in total

1.  Separation and Purification of Flavonoids from Black Currant Leaves by High-Speed Countercurrent Chromatography and Preparative HPLC.

Authors:  Dajun He; Yun Huang; Amatjan Ayupbek; Dongyu Gu; Yi Yang; Haji Akber Aisa; Yoichiro Ito
Journal:  J Liq Chromatogr Relat Technol       Date:  2010-03-01       Impact factor: 1.312

2.  Separation and purification of triterpene saponins from roots of Radix phytolaccae by high-speed countercurrent chromatography coupled with evaporative light scattering detection.

Authors:  Jie Ma; Qianliang Chen; Daowan Lai; Wenji Sun; Tianyou Zhang; Yoichiro Ito
Journal:  J Liq Chromatogr Relat Technol       Date:  2010-01-01       Impact factor: 1.312

3.  PREPARATIVE ISOLATION AND PURIFICATION OF FOUR FLAVONOIDS FROM DAPHNE GENKWA SIEB. ET ZUCC. BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY.

Authors:  Huichun Xie; Yong Liang; Yoichiro Ito; Xiaohong Wang; Renshan Chen; Juanting He; Hang Li; Tianyou Zhang
Journal:  J Liq Chromatogr Relat Technol       Date:  2011       Impact factor: 1.312

4.  PREPARATIVE ISOLATION AND PURIFICATION OF FIVE FLAVONOIDS FROM POGOSTEMON CABLIN BENTH BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY AND PREPARATIVE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY.

Authors:  Kang Li; Hongwu Zhang; Huichun Xie; Yong Liang; Xiaohong Wang; Yoichiro Ito
Journal:  J Liq Chromatogr Relat Technol       Date:  2011-01-01       Impact factor: 1.312

5.  Evaluation on the performance of four different column models mounted on the compact type-I coil planet centrifuge.

Authors:  Yi Yang; Dongyu Gu; Haji Akber Aisa; Yoichiro Ito
Journal:  J Chromatogr A       Date:  2010-10-26       Impact factor: 4.759

6.  SEPARATION OF THE MINOR FLAVONOLS FROM FLOS GOSSYPII BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY.

Authors:  Yi Yang; Yongxin Zhao; Dongyu Gu; Amatjan Ayupbek; Yun Huang; Jun Dou; Yoichiro Ito; Tianyou Zhang; Haji Akber Aisa
Journal:  J Liq Chromatogr Relat Technol       Date:  2010-01-01       Impact factor: 1.312

7.  Spiral Tube Assembly for High-Speed Countercurrent Chromatography: Choice of Elution Modes for Four Typical Two-Phase Solvent Systems.

Authors:  Y Ito; R Clary; J Powell; M Knight; T M Finn
Journal:  J Liq Chromatogr Relat Technol       Date:  2008-01-01       Impact factor: 1.312

Review 8.  Spiral countercurrent chromatography.

Authors:  Yoichiro Ito; Martha Knight; Thomas M Finn
Journal:  J Chromatogr Sci       Date:  2013-08       Impact factor: 1.618

9.  Development of a method to extract and purify target compounds from medicinal plants in a single step: online hyphenation of expanded bed adsorption chromatography and countercurrent chromatography.

Authors:  Yang Li; Nan Wang; Min Zhang; Yoichiro Ito; Hongyang Zhang; Yuerong Wang; Xin Guo; Ping Hu
Journal:  Anal Chem       Date:  2014-03-13       Impact factor: 6.986

10.  Centrifugal precipitation chromatography, a powerful technique for the isolation of active enzymes from tea leaves (Camellia sinensis).

Authors:  Susanne Baldermann; Peter Fleischmann; Mareike Bolten; Naoharu Watanabe; Peter Winterhalter; Yoichiro Ito
Journal:  J Chromatogr A       Date:  2009-02-05       Impact factor: 4.759
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