OBJECTIVES: The aim of this study was to develop and validate an ex vivo model for the assessment of radiofrequency (RF) ablation of the pancreas. METHODS: Porcine pancreata were used within 1 hour of sacrifice. RF was delivered to a premarked area in the center of the pancreatic head using a thermocouple-tipped multiprobe array. Four temperature presets were evaluated: 70 degrees-100 degrees C in 10 degrees increments. Immediately after ablation serial sections of the pancreatic head were cut to incorporate duodenum, portal vein, and bile duct. For each experiment, a portion of pancreatic tail was sampled as nonablated control. Hematoxylin and eosin (H&E) slides together with nicotinamide adenine dinucleotide (NADH) stained preparations were made. The NADH staining was quantified using computerized digital image recognition techniques. RESULTS: Control sections (n = 20) demonstrated normal pancreatic architecture on H&E and strong NADH staining indicating preserved tissue oxidative metabolism. RF produced a temperature-dependent destruction of parenchymal architecture (H&E) with a corresponding loss of NADH activity. There was no evidence of thermal injury to the duodenum. Quantification of NADH staining demonstrated a median positive staining of 69.26% (55.87-97.28) for control tissue compared with 1.40% (0-7.77) for ablated pancreas (P < 0.001; Mann-Whitney U test). CONCLUSION: This study describes the development of a relatively simple, reliable, and reproducible model for evaluation of RF ablation of pancreatic parenchyma.
OBJECTIVES: The aim of this study was to develop and validate an ex vivo model for the assessment of radiofrequency (RF) ablation of the pancreas. METHODS: Porcine pancreata were used within 1 hour of sacrifice. RF was delivered to a premarked area in the center of the pancreatic head using a thermocouple-tipped multiprobe array. Four temperature presets were evaluated: 70 degrees-100 degrees C in 10 degrees increments. Immediately after ablation serial sections of the pancreatic head were cut to incorporate duodenum, portal vein, and bile duct. For each experiment, a portion of pancreatic tail was sampled as nonablated control. Hematoxylin and eosin (H&E) slides together with nicotinamide adenine dinucleotide (NADH) stained preparations were made. The NADH staining was quantified using computerized digital image recognition techniques. RESULTS: Control sections (n = 20) demonstrated normal pancreatic architecture on H&E and strong NADH staining indicating preserved tissue oxidative metabolism. RF produced a temperature-dependent destruction of parenchymal architecture (H&E) with a corresponding loss of NADH activity. There was no evidence of thermal injury to the duodenum. Quantification of NADH staining demonstrated a median positive staining of 69.26% (55.87-97.28) for control tissue compared with 1.40% (0-7.77) for ablated pancreas (P < 0.001; Mann-Whitney U test). CONCLUSION: This study describes the development of a relatively simple, reliable, and reproducible model for evaluation of RF ablation of pancreatic parenchyma.
Authors: Tatiana D Khokhlova; Yak-Nam Wang; Julianna C Simon; Bryan W Cunitz; Frank Starr; Marla Paun; Lawrence A Crum; Michael R Bailey; Vera A Khokhlova Journal: Proc Natl Acad Sci U S A Date: 2014-05-19 Impact factor: 11.205
Authors: Avinash Eranki; Navid Farr; Ari Partanen; Karun V Sharma; Christopher T Rossi; Avi Z Rosenberg; AeRang Kim; Matthew Oetgen; Haydar Celik; David Woods; Pavel S Yarmolenko; Peter C W Kim; Bradford J Wood Journal: Int J Hyperthermia Date: 2018-02-22 Impact factor: 3.914
Authors: Matthew S Adams; Vasant A Salgaonkar; Juan Plata-Camargo; Peter D Jones; Aurea Pascal-Tenorio; Hsin-Yu Chen; Donna M Bouley; Graham Sommer; Kim Butts Pauly; Chris J Diederich Journal: Med Phys Date: 2016-07 Impact factor: 4.071
Authors: Matthew S Adams; Serena J Scott; Vasant A Salgaonkar; Graham Sommer; Chris J Diederich Journal: Int J Hyperthermia Date: 2016-01-21 Impact factor: 3.914
Authors: Muhammad Nadeem Yousaf; Hamid Ehsan; Ahmad Muneeb; Ahsan Wahab; Muhammad K Sana; Karun Neupane; Fizah S Chaudhary Journal: Front Med (Lausanne) Date: 2021-02-11