BACKGROUND: Immunohistochemical staining has been applied successfully to detect hepatitis C virus (HCV) antigen in fresh frozen tissue. In paraffin-embedded tissues, however, minimal trials with conflicting results have been reported. AIMS: The present study is a trial to evaluate the identification of HCV antigen in paraffin-embedded liver biopsies using the anti-HCV monoclonal antibody (MAb) TORDJI-22. METHODS: We applied immunohistochemical staining for HCV in 56 paraffin-embedded liver biopsy specimens, 46 from patients seropositive for HCV-RNA and 10 control liver biopsy specimens. The TORDJI-22 MAb was applied in dilution 1:40, with overnight incubation. RESULTS: Reproducible staining patterns of HCV antigen in tissues were identified among the majority (42/46-91%) of HCV RNA seropositive cases. The staining pattern was cytoplasmic of hepatocytes, with occasional nuclear hue. It is mainly coarse granular with microvesicular pattern. Three staining patterns were identified: A, diffuse or membranous; B, patchy; and C, occasional paranuclear. None of the control samples showed a similar staining pattern. CONCLUSION: Immunohistochemical identification of HCV antigen is easy to apply in paraffin-embedded liver biopsy specimens when the optimal detection techniques are applied. The staining pattern is reproducible, being mainly coarse granular cytoplasmic. Cross reactivity with hepatitis B virus antigens was not detected. Copyright Blackwell Munksgaard 2005
BACKGROUND: Immunohistochemical staining has been applied successfully to detect hepatitis C virus (HCV) antigen in fresh frozen tissue. In paraffin-embedded tissues, however, minimal trials with conflicting results have been reported. AIMS: The present study is a trial to evaluate the identification of HCV antigen in paraffin-embedded liver biopsies using the anti-HCV monoclonal antibody (MAb) TORDJI-22. METHODS: We applied immunohistochemical staining for HCV in 56 paraffin-embedded liver biopsy specimens, 46 from patients seropositive for HCV-RNA and 10 control liver biopsy specimens. The TORDJI-22 MAb was applied in dilution 1:40, with overnight incubation. RESULTS: Reproducible staining patterns of HCV antigen in tissues were identified among the majority (42/46-91%) of HCV RNA seropositive cases. The staining pattern was cytoplasmic of hepatocytes, with occasional nuclear hue. It is mainly coarse granular with microvesicular pattern. Three staining patterns were identified: A, diffuse or membranous; B, patchy; and C, occasional paranuclear. None of the control samples showed a similar staining pattern. CONCLUSION: Immunohistochemical identification of HCV antigen is easy to apply in paraffin-embedded liver biopsy specimens when the optimal detection techniques are applied. The staining pattern is reproducible, being mainly coarse granular cytoplasmic. Cross reactivity with hepatitis B virus antigens was not detected. Copyright Blackwell Munksgaard 2005
Authors: Mostafa K el-Awady; Ashraf A Tabll; Yasmine S el-Abd; Mahmoud M Bahgat; Hussein A Shoeb; Samar S Youssef; Noha G Bader el-Din; el-Rashdy M Redwan; Maha el-Demellawy; Moataza H Omran; Wael T el-Garf; Said A Goueli Journal: World J Gastroenterol Date: 2006-08-14 Impact factor: 5.742
Authors: Simon Reiss; Ilka Rebhan; Perdita Backes; Ines Romero-Brey; Holger Erfle; Petr Matula; Lars Kaderali; Marion Poenisch; Hagen Blankenburg; Marie-Sophie Hiet; Thomas Longerich; Sarah Diehl; Fidel Ramirez; Tamas Balla; Karl Rohr; Artur Kaul; Sandra Bühler; Rainer Pepperkok; Thomas Lengauer; Mario Albrecht; Roland Eils; Peter Schirmacher; Volker Lohmann; Ralf Bartenschlager Journal: Cell Host Microbe Date: 2011-01-20 Impact factor: 21.023
Authors: Mostafa K El Awady; Yasmine S El Abd; Hussein A Shoeb; Ashraf A Tabll; Alaa El Din M S Hosny; Reem M El Shenawy; Khaled Atef; Noha G Bader El Din; Mahmoud M Bahgat Journal: Virol J Date: 2006-09-01 Impact factor: 4.099