Literature DB >> 15778032

Sandwich ELISA detection of Clostridium perfringens cells and alpha-toxin from field cases of necrotic enteritis of poultry.

M T McCourt1, D A Finlay, C Laird, J A Smyth, C Bell, H J Ball.   

Abstract

Sandwich ELISAs (sELISAs) for the detection of Clostridium perfringens cells and alpha-toxin were developed and used to screen intestinal samples from normal broiler chickens and from clinical cases of necrotic enteritis. The assays clearly distinguished between the two sets of samples. The sELISA absorbance values from samples obtained from the majority of healthy birds were low and those from the majority of necrotic enteritis cases were high. Together, the assays provide a suitable test for the rapid screening for the diagnosis of necrotic enteritis in poultry.

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Year:  2005        PMID: 15778032     DOI: 10.1016/j.vetmic.2004.12.023

Source DB:  PubMed          Journal:  Vet Microbiol        ISSN: 0378-1135            Impact factor:   3.293


  5 in total

1.  Antimicrobial susceptibility of Clostridium perfringens strains isolated from broiler chickens.

Authors:  R O S Silva; F M Salvarani; R A Assis; N R S Martins; P S Pires; F C F Lobato
Journal:  Braz J Microbiol       Date:  2009-06-01       Impact factor: 2.476

2.  Dual-mode sensor based on the synergy of magnetic separation and functionalized probes for the ultrasensitive detection of Clostridium perfringens.

Authors:  Wenzhuo Wang; Wei Yuan; Debao Wang; Xutao Mai; Daoying Wang; Yongzhi Zhu; Fang Liu; Zhilan Sun
Journal:  RSC Adv       Date:  2022-09-09       Impact factor: 4.036

3.  Ultrasensitive Electrochemical Detection of Clostridium perfringens DNA Based Morphology-Dependent DNA Adsorption Properties of CeO₂ Nanorods in Dairy Products.

Authors:  Xingcan Qian; Qing Qu; Lei Li; Xin Ran; Limei Zuo; Rui Huang; Qiang Wang
Journal:  Sensors (Basel)       Date:  2018-06-08       Impact factor: 3.576

4.  Development and evaluation of indirect enzyme-linked immunosorbent assays for the determination of immune response to multiple clostridial antigens in vaccinated captive bred southern white rhinoceros (Ceratotherium simum simum).

Authors:  Angela Buys; Jannie Crafford; Henriette van Heerden
Journal:  Acta Vet Scand       Date:  2020-10-07       Impact factor: 1.695

5.  Evaluation of the efficacy of chlorogenic acid in reducing small intestine injury, oxidative stress, and inflammation in chickens challenged with Clostridium perfringens type A.

Authors:  Xinheng Zhang; Qiqi Zhao; Xiaotong Ci; Sheng Chen; Zi Xie; Hongxin Li; Huanmin Zhang; Feng Chen; Qingmei Xie
Journal:  Poult Sci       Date:  2020-10-06       Impact factor: 3.352

  5 in total

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