| Literature DB >> 15769514 |
Michiko Kaya1, Yoshiharu Toyama, Kenji Kubota, Yoshinobu Nodasaka, Masanori Ochiai, Motoyoshi Nomizu, Norio Nishi.
Abstract
Using various types of DNAs prepared from plasmid DNA, complete double-stranded DNA (ds.DNA) with linear and cyclic forms and double-stranded DNA coexisting with single-stranded DNA (ss.DNA), the structure and fibrillogenesis of the collagen-DNA complex were investigated by means of turbidity, transmission electron microscopy, and confocal laser-scanning microscopy. The rate of fibrillogenesis of the collagen-DNA complex significantly depends on the DNA structure. The structure of the fibrils formed in the complexes showed a marked difference between the ds.DNA and ss.DNA complexes with collagen. Spatial distribution of the DNA and collagen in the complexes suggests that the characteristic collagen-DNA interaction depends on the DNA forms.Entities:
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Year: 2005 PMID： 15769514 DOI： 10.1016/j.ijbiomac.2004.11.005
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953