Literature DB >> 15767699

Evaluation of hybridization conditions for spotted oligonucleotide-based DNA microarrays.

Mong-Hsun Tsai1, Hailing Yan, Xi Chen, G V R Chandramouli, Shuping Zhao, Deborah Coffin, C Norman Coleman, James B Mitchell, Eric Y Chuang.   

Abstract

We compared different hybridization conditions of oligonucleotide-based DNA microarray to acquire optimized and reliable microarray data. Several parameters were evaluated at different hybridization conditions, including signal-to-background (S:B) ratios, signal dynamic range, usable spots, and reproducibility. Statistical analysis showed that better results were obtained when spotted, presynthesized long oligonucleotide arrays were blocked with succinic anhydride and hybridized at 42 degrees C in the presence of 50% formamide.

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Year:  2005        PMID： 15767699     DOI： 10.1385/MB:29:3:221

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  8 in total

1.  Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation.

Authors:  Yee Hwa Yang; Sandrine Dudoit; Percy Luu; David M Lin; Vivian Peng; John Ngai; Terence P Speed
Journal:  Nucleic Acids Res       Date:  2002-02-15       Impact factor: 16.971

2.  DNA arrays for analysis of gene expression.

Authors:  M B Eisen; P O Brown
Journal:  Methods Enzymol       Date:  1999       Impact factor: 1.600

3.  Gene expression after treatment with hydrogen peroxide, menadione, or t-butyl hydroperoxide in breast cancer cells.

Authors:  Yao-Yu Eric Chuang; Yidong Chen; V R Chandramouli; John A Cook; Deborah Coffin; Mong-Hsun Tsai; William DeGraff; Hailing Yan; Shuping Zhao; Angelo Russo; Edison T Liu; James B Mitchell
Journal:  Cancer Res       Date:  2002-11-01       Impact factor: 12.701

4.  DNA microarrays based on noncovalent oligonucleotide attachment and hybridization in two dimensions.

Authors:  Y Belosludtsev; B Iverson; S Lemeshko; R Eggers; R Wiese; S Lee; T Powdrill; M Hogan
Journal:  Anal Biochem       Date:  2001-05-15       Impact factor: 3.365

Review 5.  Expression profiling using cDNA microarrays.

Authors:  D J Duggan; M Bittner; Y Chen; P Meltzer; J M Trent
Journal:  Nat Genet       Date:  1999-01       Impact factor: 38.330

Review 6.  Exploring the new world of the genome with DNA microarrays.

Authors:  P O Brown; D Botstein
Journal:  Nat Genet       Date:  1999-01       Impact factor: 38.330

7.  Microarray fabrication with covalent attachment of DNA using bubble jet technology.

Authors:  T Okamoto; T Suzuki; N Yamamoto
Journal:  Nat Biotechnol       Date:  2000-04       Impact factor: 54.908

8.  Quantitative monitoring of gene expression patterns with a complementary DNA microarray.

Authors:  M Schena; D Shalon; R W Davis; P O Brown
Journal:  Science       Date:  1995-10-20       Impact factor: 47.728
  8 in total
  2 in total

1.  SARS-CoV regulates immune function-related gene expression in human monocytic cells.

Authors:  Wanchung Hu; Yu-Ting Yen; Sher Singh; Chuan-Liang Kao; Betty A Wu-Hsieh
Journal:  Viral Immunol       Date:  2012-08       Impact factor: 2.257

2.  Single-step capture and sequencing of natural DNA for detection of BRCA1 mutations.

Authors:  John F Thompson; Jeffrey G Reifenberger; Eldar Giladi; Kristen Kerouac; Jaime Gill; Erik Hansen; Avak Kahvejian; Philipp Kapranov; Travis Knope; Doron Lipson; Kathleen E Steinmann; Patrice M Milos
Journal:  Genome Res       Date:  2011-07-15       Impact factor: 9.043
  2 in total

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