AIM: To define the infection status of Helicobacter pylori in 109 patients with gastric cancers and H pylori localization in gastric carcinoma tissues in South China. METHODS: The incidence of H pylori infection in gastric carcinomas was estimated by polymerase chain reaction (PCR), simultaneously; both morphological features and the localization of H pylori in gastric carcinomas were demonstrated by Warthin-Starry (WS) staining. The relationships between H pylori infection and the clinical-pathologic factors of gastric carcinomas were analyzed by software SPSS10.0. RESULTS: H pylori was found in 42 (39.03%) and 58 (53.21%) cases of 109 patients with gastric carcinomas by PCR and WS, respectively. H pylori infection rate detected in gastric carcinomas by WS was higher than that by PCR (chi2 = 9.735, P < 0.005 < 0.01). WS stain showed that H pylori existed in the gastric antrum mucus, mucosal gland of normal tissues adjacent to gastric carcinomas and the gland, mucus pool of cancer tissues. The positive rate of H pylori in normal tissues adjacent to carcinomas was higher than that in cancer tissues (chi2 = 15.750, P < 0.005 < 0.01). No significant differences in age, sex, site, histological types and lymph node metastasis were found between H pylori-positive gastric carcinomas and H pylori-negative cases by both methods, but there were statistically significant differences of H pylori positive rate between early and advanced stage of gastric carcinomas (chi2 = 4.548 or 5.922, P = 0.033 or 0.015 < 0.05). CONCLUSION: These results suggested that H pylori infection might play a certain role in the early stage of carcinogenesis of human gastric mucosa epithelia.
AIM: To define the infection status of Helicobacter pylori in 109 patients with gastric cancers and H pylori localization in gastric carcinoma tissues in South China. METHODS: The incidence of H pylori infection in gastric carcinomas was estimated by polymerase chain reaction (PCR), simultaneously; both morphological features and the localization of H pylori in gastric carcinomas were demonstrated by Warthin-Starry (WS) staining. The relationships between H pylori infection and the clinical-pathologic factors of gastric carcinomas were analyzed by software SPSS10.0. RESULTS: H pylori was found in 42 (39.03%) and 58 (53.21%) cases of 109 patients with gastric carcinomas by PCR and WS, respectively. H pylori infection rate detected in gastric carcinomas by WS was higher than that by PCR (chi2 = 9.735, P < 0.005 < 0.01). WS stain showed that H pylori existed in the gastric antrum mucus, mucosal gland of normal tissues adjacent to gastric carcinomas and the gland, mucus pool of cancer tissues. The positive rate of H pylori in normal tissues adjacent to carcinomas was higher than that in cancer tissues (chi2 = 15.750, P < 0.005 < 0.01). No significant differences in age, sex, site, histological types and lymph node metastasis were found between H pylori-positive gastric carcinomas and H pylori-negative cases by both methods, but there were statistically significant differences of H pylori positive rate between early and advanced stage of gastric carcinomas (chi2 = 4.548 or 5.922, P = 0.033 or 0.015 < 0.05). CONCLUSION: These results suggested that H pylori infection might play a certain role in the early stage of carcinogenesis of humangastric mucosa epithelia.
Authors: H Yamagata; Y Kiyohara; K Aoyagi; I Kato; H Iwamoto; K Nakayama; H Shimizu; Y Tanizaki; H Arima; N Shinohara; H Kondo; T Matsumoto; M Fujishima Journal: Arch Intern Med Date: 2000-07-10
Authors: M Inoue; K Tajima; A Matsuura; T Suzuki; T Nakamura; K Ohashi; S Nakamura; S Tominaga Journal: Cancer Lett Date: 2000-12-08 Impact factor: 8.679
Authors: Valerie P O'Brien; Amanda L Koehne; Julien Dubrulle; Armando E Rodriguez; Christina K Leverich; V Paul Kong; Jean S Campbell; Robert H Pierce; James R Goldenring; Eunyoung Choi; Nina R Salama Journal: Life Sci Alliance Date: 2020-12-11