Literature DB >> 15756459

A straight-forward method of optimising protein solubility for NMR.

Peter W A Howe1.   

Abstract

Maximising solubility is a key step in applying solution-state NMR to proteins. The 'microbatch' crystallisation screening method can be adapted to screen for protein solubility. In this approach, drops of test solutions are placed under paraffin oil in 96-well screening plates. This requires very small amounts of protein, is easy to set up and is readily automatable.

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Year:  2004        PMID： 15756459     DOI： 10.1007/s10858-004-3739-4

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  5 in total

Review 1.  Optimization of protein solubility and stability for protein nuclear magnetic resonance.

Authors:  S Bagby; K I Tong; M Ikura
Journal:  Methods Enzymol       Date:  2001       Impact factor: 1.600

2.  Compatibility of detergents with the microbatch-under-oil crystallization method.

Authors:  Patrick J Loll; Anna Tretiakova; Erik Soderblom
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2003-05-23

3.  Microdrop screening: a rapid method to optimize solvent conditions for NMR spectroscopy of proteins.

Authors:  C A Lepre; J M Moore
Journal:  J Biomol NMR       Date:  1998-11       Impact factor: 2.835

4.  The button test: a small scale method using microdialysis cells for assessing protein solubility at concentrations suitable for NMR.

Authors:  S Bagby; K I Tong; D Liu; J R Alattia; M Ikura
Journal:  J Biomol NMR       Date:  1997-10       Impact factor: 2.835

5.  Common themes in redox chemistry emerge from the X-ray structure of oilseed rape (Brassica napus) enoyl acyl carrier protein reductase.

Authors:  J B Rafferty; J W Simon; C Baldock; P J Artymiuk; P J Baker; A R Stuitje; A R Slabas; D W Rice
Journal:  Structure       Date:  1995-09-15       Impact factor: 5.006
  5 in total
  3 in total

1.  High-throughput screening of optimal solution conditions for structural biological studies by fluorescence correlation spectroscopy.

Authors:  Toshihiko Sugiki; Chie Yoshiura; Yutaka Kofuku; Takumi Ueda; Ichio Shimada; Hideo Takahashi
Journal:  Protein Sci       Date:  2009-05       Impact factor: 6.725

2.  Prediction of protein solubility from calculation of transfer free energy.

Authors:  Harianto Tjong; Huan-Xiang Zhou
Journal:  Biophys J       Date:  2008-05-30       Impact factor: 4.033

3.  Sample preparation methods to analyze DNA-induced structural changes in replication protein A.

Authors:  Chris A Brosey; Susan E Tsutakawa; Walter J Chazin
Journal:  Methods Mol Biol       Date:  2012
  3 in total

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