Literature DB >> 15750108

Loss of the mecA gene during storage of methicillin-resistant Staphylococcus aureus strains.

Arjanne van Griethuysen1, Inge van Loo, Alex van Belkum, Christina Vandenbroucke-Grauls, Wim Wannet, Peter van Keulen, Jan Kluytmans.   

Abstract

The mecA gene was lost in 36 (14.4%) of 250 methicillin-resistant Staphylococcus aureus isolates after 2 years of storage at -80 degrees C with the Microbank system (Pro-lab Diagnostics, Austin, Tex.). Further analysis of 35 of these isolates confirmed loss of the mecA gene in 32 isolates. This finding has important implications for the management of strain collections.

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Year:  2005        PMID: 15750108      PMCID: PMC1081222          DOI: 10.1128/JCM.43.3.1361-1365.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  13 in total

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Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

2.  In vivo deletion of the methicillin resistance mec region from the chromosome of Staphylococcus aureus strains.

Authors:  A Deplano; P T Tassios; Y Glupczynski; E Godfroid; M J Struelens
Journal:  J Antimicrob Chemother       Date:  2000-10       Impact factor: 5.790

3.  Multiplex PCR strategy for rapid identification of structural types and variants of the mec element in methicillin-resistant Staphylococcus aureus.

Authors:  Duarte C Oliveira; Hermínia de Lencastre
Journal:  Antimicrob Agents Chemother       Date:  2002-07       Impact factor: 5.191

4.  Survey of the methicillin resistance-associated genes mecA, mecR1-mecI, and femA-femB in clinical isolates of methicillin-resistant Staphylococcus aureus.

Authors:  R L Hürlimann-Dalel; C Ryffel; F H Kayser; B Berger-Bächi
Journal:  Antimicrob Agents Chemother       Date:  1992-12       Impact factor: 5.191

5.  Rapid and simple method for purification of nucleic acids.

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6.  Preservation of Neisseria gonorrhoeae at -20 degrees C.

Authors:  P S Harbec; P Turcotte
Journal:  J Clin Microbiol       Date:  1996-05       Impact factor: 5.948

7.  Rapid slide latex agglutination test for detection of methicillin resistance in Staphylococcus aureus.

Authors:  A van Griethuysen; M Pouw; N van Leeuwen; M Heck; P Willemse; A Buiting; J Kluytmans
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8.  Resistance of staphylococci in The Netherlands: surveillance by an electronic network during 1989-1995.

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Journal:  J Antimicrob Chemother       Date:  1998-01       Impact factor: 5.790

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10.  Consecutive isolation of homologous strains of methicillin-resistant and methicillin-susceptible Staphylococcus aureus from a hospitalized child.

Authors:  C Lawrence; M Cosseron; P Durand; Y Costa; R Leclercq
Journal:  J Hosp Infect       Date:  1996-05       Impact factor: 3.926

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  26 in total

1.  GeneXpert captures unstable methicillin-resistant Staphylococcus aureus prone to rapidly losing the mecA gene.

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2.  Performance of MRSA ID, a new chromogenic medium for detection of methicillin-resistant Staphylococcus aureus.

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4.  Evaluation of three molecular assays for rapid identification of methicillin-resistant Staphylococcus aureus.

Authors:  Patrice Francois; Manuela Bento; Gesuele Renzi; Stephan Harbarth; Didier Pittet; Jacques Schrenzel
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5.  Two-year study evaluating the potential loss of methicillin resistance in a methicillin-resistant Staphylococcus aureus culture collection.

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6.  Performance of MicroScan WalkAway and Vitek 2 for detection of oxacillin resistance in a set of methicillin-resistant Staphylococcus aureus isolates with diverse genetic backgrounds.

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7.  Effects of Frozen Storage on Detection of Intermediate Vancomycin Susceptibility and Heteroresistance in Methicillin-Resistant Staphylococcus aureus Blood Isolates.

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8.  Validation of virulence and epidemiology DNA microarray for identification and characterization of Staphylococcus aureus isolates.

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Journal:  J Clin Microbiol       Date:  2008-02-20       Impact factor: 5.948

9.  Community-associated methicillin-resistant Staphylococcus aureus isolates causing healthcare-associated infections.

Authors:  Cynthia L Maree; Robert S Daum; Susan Boyle-Vavra; Kelli Matayoshi; Loren G Miller
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10.  Performance of Oxoid Brilliance MRSA medium for detection of methicillin-resistant Staphylococcus aureus: an in vitro study.

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