Literature DB >> 15746370

Heterologous expression, purification, and characterization of a highly active xylose reductase from Neurospora crassa.

Ryan Woodyer1, Michael Simurdiak, Wilfred A van der Donk, Huimin Zhao.   

Abstract

A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol.

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Year:  2005        PMID： 15746370      PMCID： PMC1065158          DOI： 10.1128/AEM.71.3.1642-1647.2005

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  28 in total

1.  Structure of a voltage-dependent K+ channel beta subunit.

Authors:  J M Gulbis; S Mann; R MacKinnon
Journal:  Cell       Date:  1999-06-25       Impact factor: 41.582

2.  Exploring the active site of yeast xylose reductase by site-directed mutagenesis of sequence motifs characteristic of two dehydrogenase/reductase family types.

Authors:  M Klimacek; M Szekely; R Griessler; B Nidetzky
Journal:  FEBS Lett       Date:  2001-07-06       Impact factor: 4.124

Review 3.  Metabolic engineering of Saccharomyces cerevisiae for xylose utilization.

Authors:  B Hahn-Hägerdal; C F Wahlbom; M Gárdonyi; W H van Zyl; R R Cordero Otero; L J Jönsson
Journal:  Adv Biochem Eng Biotechnol       Date:  2001       Impact factor: 2.635

4.  Strategies to an efficient enzymatic production of xylitol.

Authors:  B Nidetzky; W Neuhauser; P Mayr; D Haltrich; K D Kulbe
Journal:  Ann N Y Acad Sci       Date:  1998-12-13       Impact factor: 5.691

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Purification, kinetic characterization and involvement of tryptophan residue at the NADPH binding site of xylose reductase from Neurospora crassa.

Authors:  U B Rawat; M B Rao
Journal:  Biochim Biophys Acta       Date:  1996-04-16

7.  Xylose utilisation: cloning and characterisation of the Xylose reductase from Candida tenuis.

Authors:  B Häcker; A Habenicht; M Kiess; R Mattes
Journal:  Biol Chem       Date:  1999-12       Impact factor: 3.915

8.  Continuous enzymatic production of xylitol with simultaneous coenzyme regeneration in a charged membrane reactor.

Authors:  B Nidetzky; W Neuhauser; D Haltrich; K D Kulbe
Journal:  Biotechnol Bioeng       Date:  1996-11-05       Impact factor: 4.530

9.  Multiple forms of xylose reductase in Candida intermedia: comparison of their functional properties using quantitative structure-activity relationships, steady-state kinetic analysis, and pH studies.

Authors:  Bernd Nidetzky; Kaspar Brüggler; Regina Kratzer; Peter Mayr
Journal:  J Agric Food Chem       Date:  2003-12-31       Impact factor: 5.279

10.  Purification and partial characterization of an aldo-keto reductase from Saccharomyces cerevisiae.

Authors:  A Kuhn; C van Zyl; A van Tonder; B A Prior
Journal:  Appl Environ Microbiol       Date:  1995-04       Impact factor: 4.792
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  20 in total

1.  Biochemical characterization of an L-Xylulose reductase from Neurospora crassa.

Authors:  Nikhil Nair; Huimin Zhao
Journal:  Appl Environ Microbiol       Date:  2007-01-19       Impact factor: 4.792

2.  Increasing reducing power output (NADH) of glucose catabolism for reduction of xylose to xylitol by genetically engineered Escherichia coli AI05.

Authors:  Andrew Iverson; Erin Garza; Jinfang Zhao; Yongze Wang; Xiao Zhao; Jinhua Wang; Ryan Manow; Shengde Zhou
Journal:  World J Microbiol Biotechnol       Date:  2013-02-23       Impact factor: 3.312

3.  Identification of a xylose reductase gene in the xylose metabolic pathway of Kluyveromyces marxianus NBRC1777.

Authors:  Biao Zhang; Ling Zhang; Dongmei Wang; Xiaolian Gao; Jiong Hong
Journal:  J Ind Microbiol Biotechnol       Date:  2011-06-04       Impact factor: 3.346

4.  A genetic overhaul of Saccharomyces cerevisiae 424A(LNH-ST) to improve xylose fermentation.

Authors:  Aloke K Bera; Nancy W Y Ho; Aftab Khan; Miroslav Sedlak
Journal:  J Ind Microbiol Biotechnol       Date:  2010-08-17       Impact factor: 3.346

5.  Overexpression and simple purification of the Thermotoga maritima 6-phosphogluconate dehydrogenase in Escherichia coli and its application for NADPH regeneration.

Authors:  Yiran Wang; Y-H Percival Zhang
Journal:  Microb Cell Fact       Date:  2009-06-04       Impact factor: 5.328

6.  Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from Candida tropicalis.

Authors:  Li Chun Chen; Sheng Cih Huang; Phimonphan Chuankhayan; Chung Der Chen; Yen Chieh Huang; Jeyaraman Jeyakanthan; Hsiao Fang Pang; Lee Chung Men; Yu Ching Chen; Yu Kuo Wang; Ming Yih Liu; Tung Kung Wu; Chun Jung Chen
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-03-26

7.  Understanding Functional Roles of Native Pentose-Specific Transporters for Activating Dormant Pentose Metabolism in Yarrowia lipolytica.

Authors:  Seunghyun Ryu; Cong T Trinh
Journal:  Appl Environ Microbiol       Date:  2018-01-17       Impact factor: 4.792

8.  Cloning, expression, and characterization of xylose reductase with higher activity from Candida tropicalis.

Authors:  Feiwei Zhang; Dairong Qiao; Hui Xu; Chong Liao; Shilin Li; Yi Cao
Journal:  J Microbiol       Date:  2009-06-26       Impact factor: 3.422

9.  Xylitol production is increased by expression of codon-optimized Neurospora crassa xylose reductase gene in Candida tropicalis.

Authors:  Woo Young Jeon; Byoung Hoon Yoon; Byoung Sam Ko; Woo Yong Shim; Jung Hoe Kim
Journal:  Bioprocess Biosyst Eng       Date:  2011-09-16       Impact factor: 3.210

10.  Cell-free protein synthesis energized by slowly-metabolized maltodextrin.

Authors:  Yiran Wang; Y-H Percival Zhang
Journal:  BMC Biotechnol       Date:  2009-06-28       Impact factor: 2.563
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