BACKGROUND: Helicobacter pylori infection has protean effects on gene expression in the host gastric mucosa, which have been investigated by gene chip analysis in vitro. In this study the effects of H. pylori infection on host gene expression in the gastric antral mucosa in patients were examined. METHODS: One gastric antral biopsy was obtained from a total of 18 untreated patients undergoing routine endoscopic evaluation of chronic abdominal complaints. Nine patients had histologic evidence of H. pylori infection and 9 age- and sex-matched patients had no histologic evidence of H. pylori infection. A microarray analysis was performed using a gene chip containing 35,000 human expressed sequence tags on RNA extracted from endoscopic, gastric antral biopsies, and average gene expression among infected and uninfected patients was compared. RESULTS: Underexpressed genes in infected patients' mucosa included gastric intrinsic factor and several metallothionein isoforms. Overexpressed genes in infected patients' mucosa comprised MHC Class II molecules, immunoglobulin and B-cell activation genes, as well as genes known to induce apoptosis. Changes in expression were confirmed for a subset of genes by SYBR green real-time PCR. CONCLUSIONS: Microarray analysis of antral biopsies from patients with and without H. pylori infection revealed differential expression of metal regulatory, immunity and inflammation-related genes.
BACKGROUND:Helicobacter pyloriinfection has protean effects on gene expression in the host gastric mucosa, which have been investigated by gene chip analysis in vitro. In this study the effects of H. pyloriinfection on host gene expression in the gastric antral mucosa in patients were examined. METHODS: One gastric antral biopsy was obtained from a total of 18 untreated patients undergoing routine endoscopic evaluation of chronic abdominal complaints. Nine patients had histologic evidence of H. pyloriinfection and 9 age- and sex-matched patients had no histologic evidence of H. pyloriinfection. A microarray analysis was performed using a gene chip containing 35,000 human expressed sequence tags on RNA extracted from endoscopic, gastric antral biopsies, and average gene expression among infected and uninfected patients was compared. RESULTS: Underexpressed genes in infectedpatients' mucosa included gastric intrinsic factor and several metallothionein isoforms. Overexpressed genes in infectedpatients' mucosa comprised MHC Class II molecules, immunoglobulin and B-cell activation genes, as well as genes known to induce apoptosis. Changes in expression were confirmed for a subset of genes by SYBR green real-time PCR. CONCLUSIONS: Microarray analysis of antral biopsies from patients with and without H. pyloriinfection revealed differential expression of metal regulatory, immunity and inflammation-related genes.
Authors: Wenli Liu; Ming Yan; Yueqin Liu; Ruihong Wang; Cuiling Li; Chuxia Deng; Aparna Singh; William G Coleman; Griffin P Rodgers Journal: Proc Natl Acad Sci U S A Date: 2010-06-01 Impact factor: 11.205
Authors: Hellen Dassen; Chamindie Punyadeera; Bert Delvoux; Iris Schulkens; Claudia Marchetti; Rick Kamps; Jan Klomp; Fred Dijcks; Anton de Goeij; Thomas D'Hooghe; Cleophas Kyama; Antwan Ederveen; Gerard Dunselman; Patrick Groothuis; Andrea Romano Journal: Am J Pathol Date: 2010-11 Impact factor: 4.307
Authors: Wenli Liu; Ming Yan; Yueqin Liu; Kenneth R McLeish; William G Coleman; Griffin P Rodgers Journal: J Immunol Date: 2012-07-27 Impact factor: 5.422
Authors: Zorica Zivkovic; Eliane Esteves; Consuelo Almazán; Sirlei Daffre; Ard M Nijhof; Katherine M Kocan; Frans Jongejan; José de la Fuente Journal: BMC Genomics Date: 2010-03-18 Impact factor: 3.969