| Literature DB >> 15740922 |
Cristiane da S Rauber1, Sílvia S Guterres, Elfrides E S Schapoval.
Abstract
It was the aim of this study to develop and validate a HPLC method for the quantitative determination of citral in Cymbopogon citratus volatile oil. The HPLC assay was performed using a Spherisorb CN column (250 mm x 4.6 mm, 5 microm), a n-hexane:ethanol (85:15) mobile phase and an UV detector (set at 233 nm). The following parameters were evaluated: linearity, precision, accuracy, specificity, quantification and detection limits. The method showed linearity in the range of 10.0-30.0 microg ml(-1). Precision and accuracy were determined at the concentration of 20 microg ml(-1). The concentration of citral in C. citratus volatile oil obtained with this assay was 75%. The HPLC method developed in this study showed an excellent performance (linearity, precision, accuracy and specificity) and can be applied to assay citral in volatile oil.Entities:
Mesh：
Substances：
Year: 2004 PMID： 15740922 DOI： 10.1016/j.jpba.2004.10.042
Source DB: PubMed Journal: J Pharm Biomed Anal ISSN： 0731-7085 Impact factor: 3.935