A study of reactive oxygen species in mainstream of cigarette.

UNLABELLED: Reactive oxygenated species (ROS) not only exist in living organisms, they also exist in our environment. Combustion process and photochemical reactions are the major source of environmental ROS, and combustion process produced ROS has been gradually gaining attention in recent years. The purpose of this study is to determine the concentrations of ROS in the mainstream smoke of cigarettes sold in the marketplace using the DCFH2 fluorescence method and to understand particulate and gaseous concentrations of ROS. This research will also discuss the relationship between ROS and nicotine, found in popular cigarette brands, as well as the effectiveness of cigarette filters to remove ROS. Results indicate that the ROS concentration of mainstream smoke is 18.64-54.81 nmol H2O2/l while the correlation coefficients of nicotine and tar to total ROS are 0.959 and 0.909, respectively. Gaseous ROS concentrations are 14.32-39.03 nmol H2O2/l, and make up 71.21-85.99% of the total. It can be clearly seen therefore, that ROS exist mainly in the gaseous phase. Particulate ROS is dominant at PM2.5 (ROS(TSP)/ROS(PM2.5) is 0.652-0.959). The experimental results involving the tobacco leaves and cigarette ash show that ROS in mainstream smoke comes from the combustion process and not from the tobacco leaves. There is no effective means of eliminating ROS from mainstream smoke, regardless of whether a cigarette filter contains active charcoal. PRACTICAL IMPLICATIONS: This study showed that cigarette combustion will produce high concentration of ROS, and this high concentration of ROS in mainstream cigarette smoke probably is one major factor contributing to a high incidence of lung cancer in smokers. Environmental tobacco smoke (ETS) or second-hand smoke is a major indoor air pollutant that could potentially harm non-smokers. We will try to determine the ROS in ETS in the future.