OBJECTIVES: Detection of Neisseria gonorrhoeae by commercial and in-house-based assays has been hampered by false-positive and false-negative results. The current study describes a sensitive and specific real-time 5'-nuclease PCR assay targeting a 90-bp region of the multicopy opa gene. GOAL: To evaluate the sensitivity and specificity of this assay in detection of gonococcus. STUDY: Sensitivity and specificity were determined by testing a panel of 173 microorganisms. In addition, 135 clinical samples previously evaluated by 4 nucleic acid amplification methods were also tested. RESULTS: A sensitivity of 1 copy per reaction was achieved. Positive results were only obtained for N gonorrhoeae strains including 20 cppB-negative strains. Overall, 134 of 135 clinical sample results agreed with the consensus nucleic amplification methods. CONCLUSION: This study demonstrates opa-based target can be used as an accurate and rapid PCR assay for the detection of N gonorrhoeae in clinical specimens.
OBJECTIVES: Detection of Neisseria gonorrhoeae by commercial and in-house-based assays has been hampered by false-positive and false-negative results. The current study describes a sensitive and specific real-time 5'-nuclease PCR assay targeting a 90-bp region of the multicopy opa gene. GOAL: To evaluate the sensitivity and specificity of this assay in detection of gonococcus. STUDY: Sensitivity and specificity were determined by testing a panel of 173 microorganisms. In addition, 135 clinical samples previously evaluated by 4 nucleic acid amplification methods were also tested. RESULTS: A sensitivity of 1 copy per reaction was achieved. Positive results were only obtained for N gonorrhoeae strains including 20 cppB-negative strains. Overall, 134 of 135 clinical sample results agreed with the consensus nucleic amplification methods. CONCLUSION: This study demonstrates opa-based target can be used as an accurate and rapid PCR assay for the detection of N gonorrhoeae in clinical specimens.
Authors: M Bissessor; S N Tabrizi; C K Fairley; J Danielewski; B Whitton; S Bird; S Garland; M Y Chen Journal: J Clin Microbiol Date: 2011-09-28 Impact factor: 5.948
Authors: J W Tapsall; E A Limnios; N L Nguyen; I Carter; G Lum; K Freeman; S N Tabrizi; S M Garland; D M Whiley; T P Sloots; I W Chambers Journal: J Clin Microbiol Date: 2005-04 Impact factor: 5.948
Authors: G Lum; S M Garland; S Tabrizi; G Harnett; D W Smith; T P Sloots; D M Whiley; J W Tapsall Journal: J Clin Microbiol Date: 2006-11 Impact factor: 5.948
Authors: Sepehr N Tabrizi; Magnus Unemo; Athena E Limnios; Tiffany R Hogan; Stig-Ove Hjelmevoll; Susanne M Garland; John Tapsall Journal: J Clin Microbiol Date: 2011-08-03 Impact factor: 5.948
Authors: Cameron Buckley; Ella Trembizki; Robert W Baird; Marcus Chen; Basil Donovan; Kevin Freeman; Namraj Goire; Rebecca Guy; Monica M Lahra; David Regan; David M Whiley Journal: J Clin Microbiol Date: 2015-05-20 Impact factor: 5.948
Authors: Eric P F Chow; Sepehr N Tabrizi; Samuel Phillips; David Lee; Catriona S Bradshaw; Marcus Y Chen; Christopher K Fairley Journal: J Clin Microbiol Date: 2016-07-13 Impact factor: 5.948
Authors: Johan H Melendez; Justin Hardick; Mathilda Barnes; Perry Barnes; Christopher D Geddes; Charlotte A Gaydos Journal: Sex Transm Dis Date: 2018-05 Impact factor: 2.830
Authors: H S Tunsjø; E Smedsrud; P C Holm; K Rokvam; C Schie; V Rognlien; I Augustin; A Fostervold Journal: Eur J Clin Microbiol Infect Dis Date: 2013-08-01 Impact factor: 3.267
Authors: G Lum; K Freeman; N L Nguyen; E A Limnios; S N Tabrizi; I Carter; I W Chambers; D M Whiley; T P Sloots; S M Garland; J W Tapsall Journal: Sex Transm Infect Date: 2005-10 Impact factor: 3.519