Literature DB >> 15725673

Structural and functional analysis of pTB6 from Bifidobacterium longum.

Kunihito Tanaka1, Kazuya Samura, Yasunobu Kano.   

Abstract

The complete nucleotide sequence for pTB6 (3,624 bp) from Bifidobacterium longum was determined. This plasmid is 95% homologous in nucleotide (nuc) sequence, and also 92% in RepB aa sequence, to rolling circle replication (RCR) plasmids pKJ36 and pB44, suggesting that pTB6 replicates by the rolling circle mechanism. The putative MembB, MobA, and protein encoding from orf (Orf) I detected were nonessential for plasmid replication. We constructed an immobile shuttle vector from pTB6 and pUC18, which transformed B. longum with a high efficiency of 2.5 x 10(6) transformants/microg DNA.

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Year:  2005        PMID： 15725673     DOI： 10.1271/bbb.69.422

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  15 in total

1.  Sequence analysis of two cryptic plasmids from Bifidobacterium longum DJO10A and construction of a shuttle cloning vector.

Authors:  Ju-Hoon Lee; Daniel J O'Sullivan
Journal:  Appl Environ Microbiol       Date:  2006-01       Impact factor: 4.792

Review 2.  Genomics of Actinobacteria: tracing the evolutionary history of an ancient phylum.

Authors:  Marco Ventura; Carlos Canchaya; Andreas Tauch; Govind Chandra; Gerald F Fitzgerald; Keith F Chater; Douwe van Sinderen
Journal:  Microbiol Mol Biol Rev       Date:  2007-09       Impact factor: 11.056

3.  Isolation of a minireplicon of the plasmid pG6303 of Lactobacillus plantarum G63 and characterization of the plasmid-encoded Rep replication protein.

Authors:  Jing Fan; Xuedong Xi; Yan Huang; Zhongli Cui
Journal:  J Genet       Date:  2015-06       Impact factor: 1.166

Review 4.  A Resource for Cloning and Expression Vectors Designed for Bifidobacteria: Overview of Available Tools and Biotechnological Applications.

Authors:  Lorena Ruiz; Maria Esteban-Torres; Douwe van Sinderen
Journal:  Methods Mol Biol       Date:  2021

5.  Construction of a reporter vector for the analysis of Bifidobacterium longum promoters.

Authors:  A Klijn; D Moine; M Delley; A Mercenier; F Arigoni; R D Pridmore
Journal:  Appl Environ Microbiol       Date:  2006-09-22       Impact factor: 4.792

Review 6.  Genomic insights into bifidobacteria.

Authors:  Ju-Hoon Lee; Daniel J O'Sullivan
Journal:  Microbiol Mol Biol Rev       Date:  2010-09       Impact factor: 11.056

7.  Molecular dissection of a bifidobacterial replicon.

Authors:  Michelle Cronin; Moritz Knobel; Mary O'Connell-Motherway; Gerald F Fitzgerald; Douwe van Sinderen
Journal:  Appl Environ Microbiol       Date:  2007-10-26       Impact factor: 4.792

Review 8.  Accessing the inaccessible: molecular tools for bifidobacteria.

Authors:  Zhongke Sun; Annika Baur; Daria Zhurina; Jing Yuan; Christian U Riedel
Journal:  Appl Environ Microbiol       Date:  2012-05-11       Impact factor: 4.792

9.  Development of a double-crossover markerless gene deletion system in Bifidobacterium longum: functional analysis of the α-galactosidase gene for raffinose assimilation.

Authors:  Yosuke Hirayama; Mikiyasu Sakanaka; Hidenori Fukuma; Hiroki Murayama; Yasunobu Kano; Satoru Fukiya; Atsushi Yokota
Journal:  Appl Environ Microbiol       Date:  2012-05-11       Impact factor: 4.792

10.  Improvement of bacterial transformation efficiency using plasmid artificial modification.

Authors:  Kazumasa Yasui; Yasunobu Kano; Kaori Tanaka; Kunitomo Watanabe; Mariko Shimizu-Kadota; Hirofumi Yoshikawa; Tohru Suzuki
Journal:  Nucleic Acids Res       Date:  2008-11-12       Impact factor: 16.971
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