BACKGROUND: Cidofovir and Leflunomide are used empirically in the treatment of BK virus nephropathy. The aim of this study is to quantify the antiviral activity of these drugs. METHODS: BK virus was grown in a cell-culture system. The rate of viral replication in the presence or absence of the drug being tested was assessed using a quantitative polymerase chain reaction assay. RESULTS: The inhibitory concentration, effective concentration, and selectivity index for Leflunomide are 39.7+/-6.9, 11.3+/-2.8, and 3.8+/-0.8 microg/mL, respectively. For Cidofovir, these indices were, respectively, 63.9+/-17.2, 36.3+/-11.7, and 2.3+/-0.8 microg/mL. CONCLUSIONS: The in vitro activity of Cidofovir and Leflunomide is modest, and the selectivity index is low. There is a need to develop more effective and less toxic anti-BK virus drugs for clinical use.
BACKGROUND:Cidofovir and Leflunomide are used empirically in the treatment of BK virusnephropathy. The aim of this study is to quantify the antiviral activity of these drugs. METHODS:BK virus was grown in a cell-culture system. The rate of viral replication in the presence or absence of the drug being tested was assessed using a quantitative polymerase chain reaction assay. RESULTS: The inhibitory concentration, effective concentration, and selectivity index for Leflunomide are 39.7+/-6.9, 11.3+/-2.8, and 3.8+/-0.8 microg/mL, respectively. For Cidofovir, these indices were, respectively, 63.9+/-17.2, 36.3+/-11.7, and 2.3+/-0.8 microg/mL. CONCLUSIONS: The in vitro activity of Cidofovir and Leflunomide is modest, and the selectivity index is low. There is a need to develop more effective and less toxic anti-BK virus drugs for clinical use.
Authors: Aurelio Bonavia; Michael Franti; Erin Pusateri Keaney; Kelli Kuhen; Mohindra Seepersaud; Branko Radetich; Jian Shao; Ayako Honda; Janetta Dewhurst; Kara Balabanis; James Monroe; Karen Wolff; Colin Osborne; Leanne Lanieri; Keith Hoffmaster; Jakal Amin; Judit Markovits; Michelle Broome; Elizabeth Skuba; Ivan Cornella-Taracido; Gerard Joberty; Tewis Bouwmeester; Lawrence Hamann; John A Tallarico; Ruben Tommasi; Teresa Compton; Simon M Bushell Journal: Proc Natl Acad Sci U S A Date: 2011-04-18 Impact factor: 11.205
Authors: Yijin Wang; Wenshi Wang; Lei Xu; Xinying Zhou; Ehsan Shokrollahi; Krzysztof Felczak; Luc J W van der Laan; Krzysztof W Pankiewicz; Dave Sprengers; Nicolaas J H Raat; Herold J Metselaar; Maikel P Peppelenbosch; Qiuwei Pan Journal: Antimicrob Agents Chemother Date: 2016-04-22 Impact factor: 5.191
Authors: Eva Bernhoff; Garth D Tylden; Lars J Kjerpeseth; Tore J Gutteberg; Hans H Hirsch; Christine H Rinaldo Journal: J Virol Date: 2009-12-02 Impact factor: 5.103