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Literature DB >> 15700978

Determination of the structural environment of the tyrosyl radical in prostaglandin H2 synthase-1: a high frequency ENDOR/EPR study.

John C Wilson¹, Gang Wu, Ah-lim Tsai, Gary J Gerfen.

Abstract

The catalytically active tyrosyl radical which gives rise to the "wide doublet" (WD1) signal in ovine Prostaglandin H2 Synthase-1 has been studied using high frequency (HF) pulsed ENDOR and EPR. A hydrogen-bonded deuteron was directly detected in HFENDOR (130 GHz) spectra of 1H2O/2H2O-exchanged samples. The HFENDOR spectral simulations required a distribution in hydrogen bond distances to achieve proper fits. This range of distances was consistent with that used to model the distribution in gX values detected in pulsed HFEPR spectra. Possible hydrogen-bonding partners, as well as implications regarding the mechanism of self-inactivation for PGHS, are discussed.

Entities: Chemical Disease Gene

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Year: 2005 PMID： 15700978 PMCID： PMC2855183 DOI： 10.1021/ja043853q

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

22 in total

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10 in total

1. Structural comparisons of arachidonic acid-induced radicals formed by prostaglandin H synthase-1 and -2.

Authors: Ah-lim Tsai; Gang Wu; Corina E Rogge; Jian-Ming Lü; Sheng Peng; Wilfred A van der Donk; Graham Palmer; Gary J Gerfen; Richard J Kulmacz
Journal: J Inorg Biochem Date: 2010-11-27 Impact factor: 4.155

2. Geometry of hydrogen bonds formed by lipid bilayer nitroxide probes: a high-frequency pulsed ENDOR/EPR study.

Authors: Tatyana I Smirnova; Alex I Smirnov; Serguei V Paschenko; Oleg G Poluektov
Journal: J Am Chem Soc Date: 2007-03-07 Impact factor: 15.419

3. Atomic hydrogen as high-precision field standard for high-field EPR.

Authors: Stefan Stoll; Andrew Ozarowski; R David Britt; Alexander Angerhofer
Journal: J Magn Reson Date: 2010-08-13 Impact factor: 2.229

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Authors: Corina E Rogge; Bryant Ho; Wen Liu; Richard J Kulmacz; Ah-Lim Tsai
Journal: Biochemistry Date: 2006-01-17 Impact factor: 3.162

5. Characterization of the peroxidase mechanism upon reaction of prostacyclin synthase with peracetic acid. Identification of a tyrosyl radical intermediate.

Authors: Hui-Chun Yeh; Gary J Gerfen; Jinn-Shyan Wang; Ah-Lim Tsai; Lee-Ho Wang
Journal: Biochemistry Date: 2009-02-10 Impact factor: 3.162

6. An oxyferrous heme/protein-based radical intermediate is catalytically competent in the catalase reaction of Mycobacterium tuberculosis catalase-peroxidase (KatG).

Authors: Javier Suarez; Kalina Ranguelova; Andrzej A Jarzecki; Julia Manzerova; Vladimir Krymov; Xiangbo Zhao; Shengwei Yu; Leonid Metlitsky; Gary J Gerfen; Richard S Magliozzo
Journal: J Biol Chem Date: 2009-01-12 Impact factor: 5.157

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Authors: Mohammad R Seyedsayamdost; Tomislav Argirević; Ellen C Minnihan; JoAnne Stubbe; Marina Bennati
Journal: J Am Chem Soc Date: 2009-11-04 Impact factor: 15.419