| Literature DB >> 15700468 |
Li Li1, Rong Tsao, Zhiqiang Liu, Shuying Liu, Raymond Yang, J Christopher Young, Honghui Zhu, Zeyuan Deng, Mingyong Xie, Zhihong Fu.
Abstract
Two isomeric phenylethanoid glycosides, acteoside and isoacteoside were isolated and purified from the seeds of Plantago psyllium L. for the first time by high-speed counter-current chromatography (HSCCC) using a solvent system consisting of ethyl acetate-water (1:1, v:v). By injecting 200 mg of the n-butanol extract of P. psyllium for five consecutive times, the two-step HSCCC procedure yielded a total of 165 mg of acteoside and 17.5 mg of isoacteoside from 978 mg extract. The recovery rates for acteoside and isoacteoside were 90 and 84%, respectively, and the purities were 98 and 94%, respectively. The HSCCC fractions were analyzed by HPLC and the structures were identified by UV, LC-APCI-MS in negative ion mode, and confirmed by NMR experiments.Entities:
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Year: 2005 PMID： 15700468 DOI： 10.1016/j.chroma.2004.11.024
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759