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Literature DB >> 15691978

Rapid and simple quantification of bacterial cells by using a microfluidic device.

Chieko Sakamoto¹, Nobuyasu Yamaguchi, Masao Nasu.

Abstract

This study investigated a microfluidic chip-based system (on-chip flow cytometry) for quantification of bacteria both in culture and in environmental samples. Bacterial numbers determined by this technique were similar to those obtained by direct microscopic count. The time required for this on-chip flow cytometry was only 30 min per 6 samples.

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Year: 2005 PMID： 15691978 PMCID： PMC546694 DOI： 10.1128/AEM.71.2.1117-1121.2005

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

19 in total

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Authors: A Y Fu; C Spence; A Scherer; F H Arnold; S R Quake
Journal: Nat Biotechnol Date: 1999-11 Impact factor: 54.908

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Authors: J E Hobbie; R J Daley; S Jasper
Journal: Appl Environ Microbiol Date: 1977-05 Impact factor: 4.792

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Authors: M A McClain; C T Culbertson; S C Jacobson; J M Ramsey
Journal: Anal Chem Date: 2001-11-01 Impact factor: 6.986

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Authors: R L Kepner; J R Pratt
Journal: Microbiol Rev Date: 1994-12

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Authors: D Kirchman; J Sigda; R Kapuscinski; R Mitchell
Journal: Appl Environ Microbiol Date: 1982-08 Impact factor: 4.792

11 in total

1. Dried culture spots for Xpert MTB/RIF external quality assessment: results of a phase 1 pilot study in South Africa.

Authors: L E Scott; N Gous; B E Cunningham; B D Kana; O Perovic; L Erasmus; G J Coetzee; H Koornhof; W Stevens
Journal: J Clin Microbiol Date: 2011-10-05 Impact factor: 5.948

2. Rapid, semiautomated quantification of bacterial cells in freshwater by using a microfluidic device for on-chip staining and counting.

Authors: Nobuyasu Yamaguchi; Masashi Torii; Yuko Uebayashi; Masao Nasu
Journal: Appl Environ Microbiol Date: 2010-12-17 Impact factor: 4.792

Rapid and simple quantification of bacterial cells by using a microfluidic device.

1. Continuous concentration of bacteria in a microfluidic flow cell using electrokinetic techniques.

Review 2. Micro-microchips: just how small can we go?

Review 3. Micro total analysis systems. 2. Analytical standard operations and applications.

Review 4. Fluorogenic and chromogenic substrates used in bacterial diagnostics.

Review 5. Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.

6. A microfabricated fluorescence-activated cell sorter.

7. Use of nuclepore filters for counting bacteria by fluorescence microscopy.

8. Flow cytometry of Escherichia coli on microfluidic devices.

Review 9. Use of fluorochromes for direct enumeration of total bacteria in environmental samples: past and present.

10. Statistical analysis of the direct count method for enumerating bacteria.

1. Dried culture spots for Xpert MTB/RIF external quality assessment: results of a phase 1 pilot study in South Africa.

2. Rapid, semiautomated quantification of bacterial cells in freshwater by using a microfluidic device for on-chip staining and counting.

3. Detection of waterborne parasites using field-portable and cost-effective lensfree microscopy.

4. Rapid automated cell quantification on HIV microfluidic devices.

5. Agreement, precision, and accuracy of epifluorescence microscopy methods for enumeration of total bacterial numbers.

Review 6. Fluorescence-based bioassays for the detection and evaluation of food materials.

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8. Rapid on-site monitoring of Legionella pneumophila in cooling tower water using a portable microfluidic system.

Review 9. A critical review: Recent advances in "digital" biomolecule detection with single copy sensitivity.

Review 10. Application of Biosensors for Detection of Pathogenic Food Bacteria: A Review.