PURPOSE: We recently showed that metastasis-promoting Mts1 gene (S100A4) and protein is overexpressed during progression of prostate cancer in humans. The purpose of this study was to assess the expression of S100A4 during autochthonous prostate cancer progression in transgenic adenocarcinoma of the mouse prostate (TRAMP) model. Because oral consumption of green tea polyphenols (GTP) has been shown to inhibit metastasis and prostate cancer in TRAMP, we further assessed the significance of S100A4 during chemoprevention regimen. EXPERIMENTAL DESIGN: Male TRAMP mice 8 weeks of age were equally divided into two groups. A freshly prepared 0.1% GTP solution in tap water was supplied thrice a week to experimental animals as the sole source of drinking fluid for 24 weeks, whereas the control group of animals received the same tap water throughout the experiment. The animals were sacrificed at 0, 8, 16, and 24 weeks of GTP feeding and were analyzed for S100A4 and E-cadherin. Additional untreated and treated nontransgenic controls were also included in the study. RESULTS: With the progression of age and prostate cancer growth in TRAMP mice, an increase in the expression of S100A4 at mRNA and protein level in dorsolateral prostate, but not in nontransgenic mice, occurred. GTP feeding to TRAMP mice resulted in marked inhibition of prostate cancer progression, which was associated with reduction of S100A4 and restoration of E-cadherin. CONCLUSIONS: S100A4 represents a promising marker for prostate cancer progression and could be employed as a biomarker in chemoprevention regimens.
PURPOSE: We recently showed that metastasis-promoting Mts1 gene (S100A4) and protein is overexpressed during progression of prostate cancer in humans. The purpose of this study was to assess the expression of S100A4 during autochthonous prostate cancer progression in transgenic adenocarcinoma of the mouse prostate (TRAMP) model. Because oral consumption of green tea polyphenols (GTP) has been shown to inhibit metastasis and prostate cancer in TRAMP, we further assessed the significance of S100A4 during chemoprevention regimen. EXPERIMENTAL DESIGN: Male TRAMPmice 8 weeks of age were equally divided into two groups. A freshly prepared 0.1% GTP solution in tap water was supplied thrice a week to experimental animals as the sole source of drinking fluid for 24 weeks, whereas the control group of animals received the same tap water throughout the experiment. The animals were sacrificed at 0, 8, 16, and 24 weeks of GTP feeding and were analyzed for S100A4 and E-cadherin. Additional untreated and treated nontransgenic controls were also included in the study. RESULTS: With the progression of age and prostate cancer growth in TRAMPmice, an increase in the expression of S100A4 at mRNA and protein level in dorsolateral prostate, but not in nontransgenic mice, occurred. GTP feeding to TRAMPmice resulted in marked inhibition of prostate cancer progression, which was associated with reduction of S100A4 and restoration of E-cadherin. CONCLUSIONS:S100A4 represents a promising marker for prostate cancer progression and could be employed as a biomarker in chemoprevention regimens.
Authors: Aijaz Parray; Hifzur R Siddique; Jacquelyn K Kuriger; Shrawan K Mishra; Johng S Rhim; Heather H Nelson; Hiroyuki Aburatani; Badrinath R Konety; Shahriar Koochekpour; Mohammad Saleem Journal: Int J Cancer Date: 2014-04-29 Impact factor: 7.396
Authors: Mohammad Saleem; Mee-Hyang Kweon; Jeremy James Johnson; Vaqar Mustafa Adhami; Irina Elcheva; Naghma Khan; Bilal Bin Hafeez; Kumar M R Bhat; Sami Sarfaraz; Shannon Reagan-Shaw; Vladimir S Spiegelman; Vijayasaradhi Setaluri; Hasan Mukhtar Journal: Proc Natl Acad Sci U S A Date: 2006-09-21 Impact factor: 11.205
Authors: Bilal Bin Hafeez; Vaqar Mustafa Adhami; Mohammad Asim; Imtiaz A Siddiqui; Kumar M Bhat; Weixiong Zhong; Mohammad Saleem; Maria Din; Vijayasaradhi Setaluri; Hasan Mukhtar Journal: Clin Cancer Res Date: 2009-01-15 Impact factor: 12.531
Authors: Zhong-Hua Li; Natalya G Dulyaninova; Reniqua P House; Steven C Almo; Anne R Bresnick Journal: Mol Biol Cell Date: 2010-06-02 Impact factor: 4.138
Authors: Vladimir N Malashkevich; Kristen M Varney; Sarah C Garrett; Paul T Wilder; David Knight; Thomas H Charpentier; Udupi A Ramagopal; Steven C Almo; David J Weber; Anne R Bresnick Journal: Biochemistry Date: 2008-04-15 Impact factor: 3.162
Authors: Hifzur R Siddique; Vaqar M Adhami; Aijaz Parray; Jeremy J Johnson; Imtiaz A Siddiqui; Mohammad T Shekhani; Imtiyaz Murtaza; Noona Ambartsumian; Badrinath R Konety; Hasan Mukhtar; Mohammad Saleem Journal: Genes Cancer Date: 2013-05