Literature DB >> 15662483

A loop mediated isothermal amplification (LAMP) method for detection of infectious hematopoietic necrosis virus (IHNV) in rainbow trout (Oncorhynchus mykiss).

I Gunimaladevi1, T Kono, S E Lapatra, M Sakai.   

Abstract

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) protocol was developed for detection of infectious hematopoietic necrosis virus (IHNV) RNA in rainbow trout (Oncorhynchus mykiss). A set of four primers, two outer and two inner primers for the RT-LAMP and the LAMP assay, were designed based on the sequence of G-protein of IHNV. Time and temperature conditions were optimized for 60 min at 63 degrees C for both RT-LAMP and LAMP protocols. The detection limit was found to be similar for both RT-LAMP and LAMP. When the sensitivity of RT-LAMP and LAMP were compared with conventional nested PCR, a10-fold higher sensitivity was seen for the LAMP protocols.

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Year:  2005        PMID： 15662483     DOI： 10.1007/s00705-004-0468-7

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  8 in total

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4.  Simple and rapid detection of human enterovirus 71 by reverse-transcription and loop-mediated isothermal amplification: cryopreservation affected the detection ability.

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Review 6.  Colorimetric biosensors for point-of-care virus detections.

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Review 7.  Loop-mediated isothermal amplification: an emerging technology for detection of fish and shellfish pathogens.

Authors:  R Savan; T Kono; T Itami; M Sakai
Journal:  J Fish Dis       Date:  2005-10       Impact factor: 2.767

8.  Development and evaluation of reverse transcription loop-mediated isothermal amplification assay for the detection of the fathead minnow nidovirus.

Authors:  Qingli Zhang; Isaac Standish; Andrew D Winters; Corey Puzach; Rachel Ulferts; John Ziebuhr; Mohamed Faisal
Journal:  J Virol Methods       Date:  2014-03-01       Impact factor: 2.014
  8 in total

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