Literature DB >> 15661175

Calcium ions affect the hepatitis B virus core assembly.

Yongwook Choi1, Sung Gyoo Park, Jun-Hi Yoo, Guhung Jung.   

Abstract

Previous report showed that cytosolic Ca2+ induced by hepatitis B virus X protein (HBx) promotes HBV replication. In this study, in vitro experiments showed that (i) HBV core assembly in vitro was promoted by Ca2+ through the sucrose density gradient and the analytical ultracentrifuge analysis. Also, (ii) transmission electron microscope analysis demonstrated these assembled HBV core particles were the capsids. Ex vivo experiments showed that the treatment of BAPTA-AM and cyclosporine A (CsA) reduced HBV capsids in the transfected HepG2 cells. In addition to that, the treatment of Thapsigargin (TG) increased HBV capsids in the transfected HepG2 cells. Furthermore, we investigated the increased HBV core assembly by HBx. The results show that the increased cytosolic calcium ions by HBx promote the HBV core assembly.

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Year:  2005        PMID： 15661175     DOI： 10.1016/j.virol.2004.11.019

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  31 in total

1.  Classical nucleation theory of virus capsids.

Authors:  Roya Zandi; Paul van der Schoot; David Reguera; Willem Kegel; Howard Reiss
Journal:  Biophys J       Date:  2005-12-30       Impact factor: 4.033

Review 2.  Calcium signaling in the liver.

Authors:  Maria Jimena Amaya; Michael H Nathanson
Journal:  Compr Physiol       Date:  2013-01       Impact factor: 9.090

3.  Production of infectious human cytomegalovirus virions is inhibited by drugs that disrupt calcium homeostasis in the endoplasmic reticulum.

Authors:  Jennifer A Isler; Tobi Goldberg Maguire; James C Alwine
Journal:  J Virol       Date:  2005-12       Impact factor: 5.103

4.  Comprehensive Proteomics Identification of IFN-λ3-regulated Antiviral Proteins in HBV-transfected Cells.

Authors:  Jiradej Makjaroen; Poorichaya Somparn; Kenneth Hodge; Witthaya Poomipak; Nattiya Hirankarn; Trairak Pisitkun
Journal:  Mol Cell Proteomics       Date:  2018-08-10       Impact factor: 5.911

5.  The hepatitis B virus X protein elevates cytosolic calcium signals by modulating mitochondrial calcium uptake.

Authors:  Bei Yang; Michael J Bouchard
Journal:  J Virol       Date:  2011-10-26       Impact factor: 5.103

6.  Phosphorylation of hepatitis B virus Cp at Ser87 facilitates core assembly.

Authors:  Hee Yong Kang; Seungkeun Lee; Sung Gyoo Park; Jaehoon Yu; Youngsoo Kim; Guhung Jung
Journal:  Biochem J       Date:  2006-09-01       Impact factor: 3.857

7.  Hepatitis B Virus X Protein Upregulates Intracellular Calcium Signaling by Binding C-terminal of Orail Protein.

Authors:  Jing-Hong Yao; Zi-Jian Liu; Jian-Hua Yi; Jun Wang; Ya-Nan Liu
Journal:  Curr Med Sci       Date:  2018-03-15

8.  The L60V variation in hepatitis B virus core protein elicits new epitope-specific cytotoxic T lymphocytes and enhances viral replication.

Authors:  Yu Zhang; Yulin Ren; Yan Wu; Bao Zhao; Lipeng Qiu; Xiaodong Li; Dongping Xu; Jun Liu; George F Gao; Songdong Meng
Journal:  J Virol       Date:  2013-05-15       Impact factor: 5.103

9.  Conformational equilibria and rates of localized motion within hepatitis B virus capsids.

Authors:  Jonathan K Hilmer; Adam Zlotnick; Brian Bothner
Journal:  J Mol Biol       Date:  2007-10-22       Impact factor: 5.469

Review 10.  Assembly and Release of Hepatitis B Virus.

Authors:  Lisa Selzer; Adam Zlotnick
Journal:  Cold Spring Harb Perspect Med       Date:  2015-11-09       Impact factor: 6.915
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