| Literature DB >> 15644190 |
Fabrice A Kolb1, Haidi Zhang, Kasia Jaronczyk, Nasser Tahbaz, Tom C Hobman, Witold Filipowicz.
Abstract
Dicer is a multidomain ribonuclease that processes double-stranded RNAs (dsRNAs) to 21-nt small interfering RNAs (siRNAs) during RNA interference and excises microRNAs (miRNAs) from precursor hairpins. PAZ and PIWI domain (PPD) proteins, also involved in RNAi and miRNA function, are the best-characterized proteins known to interact with Dicer. PPD proteins are the core constituents of effector complexes, RISCs and miRNPs, mediating siRNA and miRNA function. In this chapter we describe overexpression and purification of recombinant human Dicer, its biochemical properties, and mapping of domains responsible for Dicer-PPD protein interactions.Entities:
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Year: 2005 PMID： 15644190 DOI： 10.1016/S0076-6879(04)92019-8
Source DB: PubMed Journal: Methods Enzymol ISSN： 0076-6879 Impact factor: 1.600