| Literature DB >> 15639338 |
Jiøina Hofmanová1, Alena Vaculová, Alois Kozubík.
Abstract
The proliferative and apoptotic response to TNF-alpha and anti-Fas antibody (CH-11) in human colon adenocarcinoma HT-29 cells was modulated by pretreatment with arachidonic (AA, 20:4, n-6) or docosahexaenoic (DHA, 22:6, n-3) fatty acids, which alone increased reactive oxygen species production and lipid peroxidation, and decreased the S-phase of the cell cycle. The higher amount of floating cells, subG0/G1 population and apoptotic cells detected in pre-treated cells was potentiated by cycloheximide. The effects of CH-11 were associated with activation of caspase-8, -9, and -3, cleavage of poly(ADP-ribose)polymerase-PARP, and decreased mitochondrial membrane potential (MMP), but these parameters were not significantly changed after PUFA pretreatment.Entities:
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Year: 2005 PMID： 15639338 DOI： 10.1016/j.canlet.2004.07.038
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679