Literature DB >> 15622864

Real-time detection of polymerase activity using supercritical angle fluorescence.

Alexander Krieg1, Thomas Ruckstuhl, Stephan Laib, Stefan Seeger.   

Abstract

We investigated the incorporation efficiencies of different fluorescently labelled dNTPs with polymerases by complementary strand synthesis. For this reason single stranded DNA was immobilized on a coverslip and the increase of fluorescence due to the synthesis of the corresponding strand with tagged dNTPs was detected with a supercritical angle fluorescence biosensor in real-time. By comparison of the observed signal intensities it was possible to conclude that the system Cy5-dCTP-Klenow (exonuclease free) fragment gives the best incorporation yield of the investigated enzymes and dNTPs.

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Year:  2004        PMID： 15622864     DOI： 10.1023/b:jofl.0000014663.24696.27

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.217


  17 in total

1.  Comparison between different strategies of covalent attachment of DNA to glass surfaces to build DNA microarrays.

Authors:  N Zammatteo; L Jeanmart; S Hamels; S Courtois; P Louette; L Hevesi; J Remacle
Journal:  Anal Biochem       Date:  2000-04-10       Impact factor: 3.365

2.  Fluorescence-based DNA polymerase assay.

Authors:  H Tveit; T Kristensen
Journal:  Anal Biochem       Date:  2001-02-01       Impact factor: 3.365

3.  The large fragment of Escherichia coli DNA polymerase I can synthesize DNA exclusively from fluorescently labeled nucleotides.

Authors:  S Brakmann; P Nieckchen
Journal:  Chembiochem       Date:  2001-10-01       Impact factor: 3.164

Review 4.  Discovery of superior enzymes by directed molecular evolution.

Authors:  S Brakmann
Journal:  Chembiochem       Date:  2001-12-03       Impact factor: 3.164

Review 5.  A review of DNA sequencing techniques.

Authors:  Lilian T C França; Emanuel Carrilho; Tarso B L Kist
Journal:  Q Rev Biophys       Date:  2002-05       Impact factor: 5.318

6.  Zero-mode waveguides for single-molecule analysis at high concentrations.

Authors:  M J Levene; J Korlach; S W Turner; M Foquet; H G Craighead; W W Webb
Journal:  Science       Date:  2003-01-31       Impact factor: 47.728

7.  Fluorescence spectral properties of cyanine dye labeled DNA near metallic silver particles.

Authors:  Joanna Malicka; Ignacy Gryczynski; Badri P Maliwal; Jiyu Fang; Joseph R Lakowicz
Journal:  Biopolymers       Date:  2003       Impact factor: 2.505

8.  Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. II. High-density labeling of natural DNA.

Authors:  Taurai Tasara; Bernhard Angerer; Martine Damond; Holger Winter; Sabine Dörhöfer; Ulrich Hübscher; Mario Amacker
Journal:  Nucleic Acids Res       Date:  2003-05-15       Impact factor: 16.971

9.  Incorporation of reporter molecule-labeled nucleotides by DNA polymerases. I. Chemical synthesis of various reporter group-labeled 2'-deoxyribonucleoside-5'-triphosphates.

Authors:  Gerald Giller; Taurai Tasara; Bernhard Angerer; Klaus Mühlegger; Mario Amacker; Holger Winter
Journal:  Nucleic Acids Res       Date:  2003-05-15       Impact factor: 16.971

10.  Real-time detection of nucleotide incorporation during complementary DNA strand synthesis.

Authors:  Alexander Krieg; Stephan Laib; Thomas Ruckstuhl; Stefan Seeger
Journal:  Chembiochem       Date:  2003-07-07       Impact factor: 3.164
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