Near-infrared (NIR) spectroscopy for the non-destructive and fast determination of geographical origin of Angelicae gigantis Radix.

Near-infrared (NIR) spectroscopy has been utilized to discriminate the Korean and Chinese Angelicae gigantis Radix. Decursin, one of the major ingredients in Korean Angelicae gigantis Radix, was preliminarily identified using TLC and HPLC. Decursin was then used as a unique marker component for successful discrimination between two geographical origins. Second derivative spectra were used to reduce baseline variations observed in original diffuse reflectance spectra as well as to enhance spectral features. The unique 1625 nm band of decursin in Korean samples provided clear spectral differences over Chinese samples. To develop a calibration model, soft independent modeling of class analogy (SIMCA) was used. With the use of the SIMCA model, independent sample datasets collected at two different periods were predicted. The Korean and Chinese samples were clearly identified with 100% accuracy.