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Literature DB >> 15617384

Fluorescence enhancements on silver colloid coated surfaces.

Joanna Lukomska¹, Joanna Malicka, Ignacy Gryczynski, Joseph R Lakowicz.

Abstract

We observed a strong, more than 16-fold, enhancement of Texas Red-labeled BSA fluorescence emission when deposited on silver colloid coated surfaces (SCCS). The same labeled protein deposited on silver island films (SIFs) showed an approximate 8-fold fluorescence enhancement. The lifetimes of Texas Red-BSA fluorescence are significantly shorter on silvered surfaces than on uncoated quartz substrate indicating a strong change in radiative decay rate of the dyes. We also observed a 36-fold increased brightness of overlabeled fluorescein-HSA deposited on silver colloid coated surface. Stronger enhancement observed for overlabeled Fl-HSA protein indicates that presence of silver particles partially decreased self-quenching. Our results indicate that surfaces coated with silver colloids are valuable substrates for metal-enhanced fluorescence.

Entities: Chemical

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Year: 2004 PMID： 15617384 PMCID： PMC2737396 DOI： 10.1023/b:jofl.0000031823.04926.75

Source DB: PubMed Journal: J Fluoresc ISSN： 1053-0509 Impact factor: 2.217

13 in total

1. Radiative decay engineering. 2. Effects of Silver Island films on fluorescence intensity, lifetimes, and resonance energy transfer.

Authors: Joseph R Lakowicz; Yibing Shen; Sabato D'Auria; Joanna Malicka; Jiyu Fang; Zygmunt Gryczynski; Ignacy Gryczynski
Journal: Anal Biochem Date: 2002-02-15 Impact factor: 3.365

10. Large Fluorescence Enhancements of Fluorophore Ensembles with Multilayer Plasmonic Substrates: Comparison of Theory and Experimental Results.

Authors: Henryk Szmacinski; Ramachandram Badugu; Farhad Mahdavi; Steve Blair; Joseph R Lakowicz
Journal: J Phys Chem C Nanomater Interfaces Date: 2012-10-11 Impact factor: 4.126

Fluorescence enhancements on silver colloid coated surfaces.

1. Radiative decay engineering. 2. Effects of Silver Island films on fluorescence intensity, lifetimes, and resonance energy transfer.

2. Fluorescence properties of labeled proteins near silver colloid surfaces.

3. Photodeposition of silver can result in metal-enhanced fluorescence.

4. DNA hybridization assays using metal-enhanced fluorescence.

5. Enhanced Fluorescence from Fluorophores on Fractal Silver Surfaces.

6. Electrochemical and Laser Deposition of Silver for Use in Metal-Enhanced Fluorescence.

7. Fluorescent lifetimes of molecules on silver-island films.

8. Effects of fluorophore-to-silver distance on the emission of cyanine-dye-labeled oligonucleotides.

9. Self-Assembled Metal Colloid Monolayers: An Approach to SERS Substrates.

10. Release of the self-quenching of fluorescence near silver metallic surfaces.

Review 1. Advances in surface-enhanced fluorescence.

2. Decreasing photobleaching by silver island films: application to muscle.

3. A novel method for monitoring monoclonal antibody production during cell culture.

4. Time-Resolved Fluorometric Method for One-Step Immunoassays Using Plasmonic Nanostructures.

5. Metal particle-enhanced fluorescent immunoassays on metal mirrors.

6. Silver nanoparticle-enhanced fluorescence in microtransponder-based immuno- and DNA hybridization assays.

7. Fluorescence enhancement of fluorophores tethered to different sized silver colloids deposited on glass substrate.

8. Depolarization of surface-enhanced fluorescence: an approach to fluorescence polarization assays.

9. Metal Enhanced Fluorescence on Silicon Wafer Substrates.

10. Large Fluorescence Enhancements of Fluorophore Ensembles with Multilayer Plasmonic Substrates: Comparison of Theory and Experimental Results.