| Literature DB >> 17316540 |
Evgenia G Matveeva1, Ignacy Gryczynski, Anne Barnett, Zoya Leonenko, Joseph R Lakowicz, Zygmunt Gryczynski.
Abstract
We present fluoroimmunoassays on plain metal-coated surfaces (metal mirrors) enhanced by metal nanoparticles (silver island films [SIFs]). Metal mirrors (aluminum, gold, or silver protected with a thin silica layer) were coated with SIFs, and an immunoassay (model assay for rabbit immunoglobulin G or myoglobin immunoassay) was performed on this surface using fluorescently labeled antibodies. Our results showed that SIFs alone (on glass surface not coated with metal) enhance the immunoassay signal approximately 3- to 10-fold. Using a metal mirror instead of glass as support for SIFs results in up to 50-fold signal enhancement.Entities:
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Year: 2007 PMID： 17316540 PMCID： PMC6814007 DOI： 10.1016/j.ab.2007.01.030
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365