Literature DB >> 1561080

The rat poly pyrimidine tract binding protein (PTB) interacts with a single-stranded DNA motif in a liver-specific enhancer.

P Jansen-Dürr1, M Boshart, B Lupp, A Bosserhoff, R W Frank, G Schütz.   

Abstract

We characterized and purified a protein from rat liver which specifically binds to a DNA motif present in a liver-specific enhancer of the rat tyrosine aminotransferase (TAT) gene, when offered as single-stranded DNA. Binding is highly sequence-specific and coincides with a region known to be essential for function of the enhancer. Microsequencing revealed that this protein is the rat homologue of the mouse and human poly Pyrimidine Tract binding protein (PTB), which has been shown to bind to premRNA and may participate in RNA splicing. This finding was corroborated by subsequent Western blot experiments using a PTB-specific antibody. These findings indicate a possible dual role for this protein in RNA processing and transcription.

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Year:  1992        PMID： 1561080      PMCID： PMC312165          DOI： 10.1093/nar/20.6.1243

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  24 in total

Review 1.  Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.

Authors:  P J Mitchell; R Tjian
Journal:  Science       Date:  1989-07-28       Impact factor: 47.728

2.  Cloning and sequencing of PYBP, a pyrimidine-rich specific single strand DNA-binding protein.

Authors:  F Brunel; P M Alzari; P Ferrara; M M Zakin
Journal:  Nucleic Acids Res       Date:  1991-10-11       Impact factor: 16.971

3.  Differential activation of the E2F transcription factor by the adenovirus EIa and EIV products in F9 cells.

Authors:  H Boeuf; B Reimund; P Jansen-Durr; C Kédinger
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4.  Estrogen receptor selectively binds the "coding strand" of an estrogen responsive element.

Authors:  D A Lannigan; A C Notides
Journal:  Proc Natl Acad Sci U S A       Date:  1989-02       Impact factor: 11.205

Review 5.  Enzymes of general recombination.

Authors:  M M Cox; I R Lehman
Journal:  Annu Rev Biochem       Date:  1987       Impact factor: 23.643

6.  Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes.

Authors:  D A Hager; R R Burgess
Journal:  Anal Biochem       Date:  1980-11-15       Impact factor: 3.365

7.  A 62,000 molecular weight spliceosome protein crosslinks to the intron polypyrimidine tract.

Authors:  J Wang; T Pederson
Journal:  Nucleic Acids Res       Date:  1990-10-25       Impact factor: 16.971

8.  Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns.

Authors:  M A García-Blanco; S F Jamison; P A Sharp
Journal:  Genes Dev       Date:  1989-12       Impact factor: 11.361

9.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

10.  Micropreparative separation of peptides derived from sodium dodecyl sulphate-solubilized proteins.

Authors:  A Bosserhoff; J Wallach; R W Frank
Journal:  J Chromatogr       Date:  1989-06-28
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  10 in total

1.  CD43 gene expression is mediated by a nuclear factor which binds pyrimidine-rich single-stranded DNA.

Authors:  O C Farokhzad; J M Teodoridis; H Park; M A Arnaout; C S Shelley
Journal:  Nucleic Acids Res       Date:  2000-06-01       Impact factor: 16.971

2.  The AT-rich tract of the SV40 ori core: negative synergism and specific recognition by single stranded and duplex DNA binding proteins.

Authors:  I Galli; S M Iguchi-Ariga; H Ariga
Journal:  Nucleic Acids Res       Date:  1992-07-11       Impact factor: 16.971

3.  Purification of splicing factor SF1, a heat-stable protein that functions in the assembly of a presplicing complex.

Authors:  A Krämer
Journal:  Mol Cell Biol       Date:  1992-10       Impact factor: 4.272

4.  Cloning and characterisation of a nuclear, site specific ssDNA binding protein.

Authors:  M P Smidt; B Russchen; L Snippe; J Wijnholds; G Ab
Journal:  Nucleic Acids Res       Date:  1995-07-11       Impact factor: 16.971

5.  Interaction of two sequence-specific single-stranded DNA-binding proteins with an essential region of the beta-casein gene promoter is regulated by lactogenic hormones.

Authors:  S Altiok; B Groner
Journal:  Mol Cell Biol       Date:  1993-12       Impact factor: 4.272

6.  Cloning and characterization of a single-stranded DNA binding protein that specifically recognizes deoxycytidine stretch.

Authors:  K Ito; K Sato; H Endo
Journal:  Nucleic Acids Res       Date:  1994-01-11       Impact factor: 16.971

7.  TLS/FUS (translocated in liposarcoma/fused in sarcoma) regulates target gene transcription via single-stranded DNA response elements.

Authors:  Adelene Y Tan; Todd R Riley; Tristan Coady; Harmen J Bussemaker; James L Manley
Journal:  Proc Natl Acad Sci U S A       Date:  2012-03-29       Impact factor: 11.205

8.  The polypyrimidine tract binding (PTB) protein interacts with single-stranded DNA in a sequence-specific manner.

Authors:  F Brunel; M M Zakin; H Buc; M Buckle
Journal:  Nucleic Acids Res       Date:  1996-05-01       Impact factor: 16.971

9.  A sequence-specific single-strand DNA binding protein that contacts repressor sequences in the human GM-CSF promoter.

Authors:  L S Coles; F Occhiodoro; M A Vadas; M F Shannon
Journal:  Nucleic Acids Res       Date:  1994-10-11       Impact factor: 16.971

10.  Negative regulation of the vascular smooth muscle alpha-actin gene in fibroblasts and myoblasts: disruption of enhancer function by sequence-specific single-stranded-DNA-binding proteins.

Authors:  S Sun; E S Stoflet; J G Cogan; A R Strauch; M J Getz
Journal:  Mol Cell Biol       Date:  1995-05       Impact factor: 4.272
  10 in total

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